Active Recombinant Human IL25 protein, His-tagged, Low Endotoxin

• Cat.No.: IL25-153H
• Product Overview: Recombinant Human IL25 protein(Q9H293-1)(Tyr33-Gly177), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 33-177 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human IL-25, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human IL-17RB, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 17.6 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: IL25 interleukin 25 [ Homo sapiens ]
• Official Symbol: IL25
• Synonyms: IL25; interleukin 25; IL17E, interleukin 17E; interleukin-25; IL 17E; IL 25; interleukin 17E; interleukin-17E; IL17E
• Gene ID: 64806
• mRNA Refseq: NM_022789
• Protein Refseq: NP_073626
• MIM: 605658
• UniProt ID: Q9H293