Recombinant Human Interleukin 1, Alpha, Fc Chimera

Cat.No. : IL1A-109H
Product Overview : Recombinant Human interleukin 1, alpha encoding the signal peptide and extracellular domain of human IL-1 Receptor I (IL-1RI) (aa 1-333) , was expressed in modifiedhuman 293 cells. It was fused to the Fc region of human IgG1 (aa 93-330).
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Cat. No. : IL1A -109H
Description : Interleukin 1, alpha Fc Chimera is an 80kDa type I transmembrane glycoprotein expressed on T cells and fibroblasts. Structurally the extracellular domain (ECD) of IL-1R1 contains 3 Immunoglobulin like domains. Glycosylation of IL-1R1has been shown to be necessary for optimal binding of IL-1, as blocking of glycosylation sites reduces binding to IL-1.
Source : Human 293 cells.
Amino Acid Sequence : LEADKCK EREEKIIL VS SANEIDVR PCP LNPNEHK GTITWYK DDSKTP VSTEQA SRIHQ HKEKLWF VPAKVED SGHYY CVVR NSSY CLRIK ISAK FVE NEP NL CY NAQAIFKQK LPVAGDGGLVC PYMEFFK NENN EL PK LQ W YK DCKPLLLDNI HFSG VKDRLIVM NVAEKH RGNYTCH ASYTYLG KQYPITRVIE FIT LEE NKPT RPVIVSP ANETMEVDLG SQIQLICNVTGQL SDIAYWKWNGSVIDE DDPVLGE DYYSVE NPANKR RSTLI TVLNIS EIESRFYK HPFTCFAKN THGIDAAYIQ LIYPVTNRSS NTKV DKK VEPKS CDKTH TCPPCP AP ELLGGPSV FL FPP KPK DTLMISRTPEVTCVVVDVSHEDPE VKFNWY VDGVEV HNAKTK PREEQY NSTY RVVSVL TVLHQD WLNGK EYKCR VSNKALP APIEKTISKAKGQ PREPQ YTL PPSRDE LTKNQVSLTCL VKGFYPSDIAV EWESNGQPEN NYKTTPPVLDSD GSF FLYSKL TVDKS RWQQGNV FSCSVM HEALH NHYTQKSLSLSPGK.
Molecular Mass : Interleukin 1, alpha Fc Chimera migrates as a broad band between 65 and 100 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with unmodified IL-1RI-Fc that has a predicted molecular mass of 63.6 kDa.
pI : Interleukin 1, alpha Fc Chimera separates into a number of isoforms with a pI between 5 and 8 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL-1RI-Fc that has a predicted pI of 7.
% Carbohydrate : Purified Interleukin 1, alpha Fc Chimera consists of 0-36% carbohydrate by weight.
Glycosylation : Interleukin 1, alpha Fc Chimera has N-linked and may have O-linked oligosaccharides.
Purity : >95%, as determined by SDS-PAGE and visualized by silver stain.
Formulation : When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution : It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Storage : Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Gene Name : IL1A interleukin 1, alpha [ Homo sapiens ]
Synonyms : IL1A; interleukin 1, alpha; IL1; IL-1A; IL1F1; IL1-ALPHA; preinterleukin 1 alpha; hematopoietin-1; pro-interleukin-1-alpha; Interleukin-1 alpha;IL-1 alpha; Hematopoietin-1; IL1A (IL1F1)
Gene ID : 3552
mRNA Refseq : NM_000573
Protein Refseq : NP_000566
MIM : 147760
UniProt ID : P01583
Chromosome Location : 2q14
Pathway : Apoptosis; Cytokine-cytokine receptor interaction; Graft-versus-host disease; Hematopoietic cell lineage; MAPK signaling pathway; Prion diseases; Type I diabetes mellitus
Function : cytokine activity; interleukin-1 receptor binding; protein binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What methods are employed to dissect the intricate cross-talk between IL-1α and other cytokines, shedding light on its role in immune modulation? 11/02/2021

Cross-talk between IL-1α's and other cytokines is dissected through methods such as co-immunoprecipitation, ELISA, and cytokine profiling arrays.

What experimental approaches allow researchers to dissect the paradoxical roles of IL-1α as both a pro-inflammatory and tissue repair mediator in distinct contexts? 09/22/2021

IL-1α's paradoxical roles are dissected using conditional knockout mice, 3D culture systems, and molecular analyses to delineate context-specific effects.

How are cutting-edge techniques like single-cell RNA sequencing utilized to unravel the heterogeneity in IL-1α-responsive cell populations and their functional implications? 06/07/2021

Single-cell RNA sequencing uncovers heterogeneity in IL-1α-responsive cell populations and their roles, revealing distinct molecular profiles and functions.

How is the intricate web of IL-1α's pro-inflammatory signaling pathways decoded using advanced experimental techniques? 05/26/2021

IL-1α's intricate pro-inflammatory signaling pathways are decoded using advanced techniques like mass spectrometry, phosphoproteomics, and bioinformatics.

How are state-of-the-art genetic editing tools such as CRISPR-Cas9 applied to unravel IL-1α's precise contributions to inflammatory diseases? 04/06/2021

Genetic editing tools like CRISPR-Cas9 unveil IL-1α's contributions to diseases by generating knockout models and studying resulting phenotypic changes.

How do researchers manipulate in vitro co-culture systems to dissect the dynamic interplay between immune cells and stromal cells in IL-1α-mediated responses? 06/18/2020

In vitro co-culture systems are manipulated to study IL-1α-mediated responses, elucidating immune-stromal cell interactions via cytokine-specific neutralization.

What experimental strategies enable the identification of specific cellular receptors and downstream effectors responsible for IL-1α's diverse physiological effects? 03/19/2020

Specific cellular receptors and effectors influenced by IL-1α are identified through techniques like co-immunoprecipitation, ChIP-seq, and siRNA knockdown.

How are sophisticated imaging techniques like live-cell microscopy harnessed to capture the real-time dynamics of IL-1α release and its impact on neighboring cells? 12/02/2019

Live-cell microscopy captures real-time IL-1α release dynamics and its impact on neighboring cells, facilitated by fluorescent tagging and high-resolution imaging.

How is the quantitative relationship between IL-1α levels and disease progression established through longitudinal studies and statistical modeling? 07/15/2018

Longitudinal studies and statistical modeling establish the quantitative relationship between IL-1α levels and disease progression, offering predictive insights.

How do multi-omics analyses, including transcriptomics and proteomics, provide a holistic view of the network of molecules influenced by IL-1α signaling? 06/04/2018

Multi-omics analyses provide a holistic view of IL-1α signaling's impact, integrating transcriptomics, proteomics, and metabolomics data for comprehensive insights.

Customer Reviews (3)

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Reviews
04/08/2021

    High solubility. -

    07/02/2020

      Good for intracellular staining. -

      03/13/2019

        Effective in apoptosis assays. -

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