Recombinant Human Interleukin 10

• Cat.No.: IL10-40H
• Product Overview: Recombinant Human Interleukin-10 produced inCHOis a single, glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18606.29 Dalton. The IL-10 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: IL10-40H
• Description: IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.
• Source: Chinese Hamster Ovary Cells
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein was lyophilized from a concentrated (1mg/ml) solution containing 60mM PBS pH-6.5, 50mg BSA and 100mM NaCl.
• Solubility: It is recommended to reconstitute the lyophilized Interleukin-10 in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED₅₀as determined by the dose-dependant co-stimulation with murine IL-4 of MC-9 cells was found to be < 2ng/ml, corresponding to a Specific Activity of 1.5 x 10⁷ IU/mg.
• Storage: Lyophilized Interleukin 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL10 should be stored at 4℃ between 2-7 days and for future use below -18℃. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: IL10 interleukin 10 [ Homo sapiens ]
• Synonyms: CSIF; TGIF; IL-10; IL10A; MGC126450; MGC126451; IL10; interleukin 10; cytokine synthesis inhibitory factor; Interleukin-10; Cytokine synthesis inhibitory factor
• Gene ID: 3586
• mRNA Refseq: NM_000572
• Protein Refseq: NP_000563
• MIM: 124092
• UniProt ID: P22301
• Chromosome Location: 1q31-q32
• Pathway: Allograft rejection; Asthma; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Systemic lupus erythematosus; T cell receptor signaling pathway
• Function: cytokine activity; protein binding ; interleukin-10 receptor binding; growth factor activity