Recombinant Human MAP/Microtubule Affinity-Regulating Kinase 2, GST-tagged

• Cat.No.: MARK2-1057H
• Product Overview: Recombinant full-length human MARK2 was expressed by baculovirus in Sf9 insect cells.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: MARK2 or microtubule affinity-regulating kinase 2 is a member of the Par-1 family of serine/threonine protein kinases. MARK2 is an important regulator of cell polarity in epithelial and neuronal cells and controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins (1). MARK2 is a key target of H. pylori CagA in the disorganization of gastric epithelial architecture underlying mucosal damage, inflammation, and carcinogenesis (2). MARK2 phosphorylates kinesin-like motor protein GAKIN/KIF13B to regulate axon formation. MARK2 function in the establishment of T cell polarity following engagement to an APC.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 114 kDa
• Expression System: Sf9 insect cells using baculovirus.
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
• Specific Activity: 795 nmol/min/mg
• Purity: > 70%
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: LKB1 signaling events; Notch signaling pathway; TNF-alpha/NF-kB Signaling Pathway; Wnt Signaling Pathway NetPath

Gene Information

• Gene Name: MARK2 MAP/microtubule affinity-regulating kinase 2 [ Homo sapiens ]
• Official Symbol: MARK2
• Synonyms: MARK2; MAP/microtubule affinity-regulating kinase 2; EMK1; EMK-1; PAR-1; Par1b; MGC99619; serine/threonine-protein kinase MARK2; PAR1 homolog; OTTHUMP00000218338; OTTHUMP00000218339; OTTHUMP00000218340; OTTHUMP00000218343; OTTHUMP00000236749; OTTHUMP00000236750; OTTHUMP00000236758; OTTHUMP00000237224; ELKL motif kinase 1; Ser/Thr protein kinase PAR-1B; serine/threonine protein kinase EMK; EC=2.7.11.1; PAR1 homolog
• Gene ID: 2011
• mRNA Refseq: NM_001039469
• Protein Refseq: NP_001034558
• MIM: 600526
• UniProt ID: Q7KZI7
• Chromosome Location: 11q13.1
• Function: ATP binding; magnesium ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity