| Description : |
The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
164.3 kDa |
| AA Sequence : |
MAAAAGNRASSSGFPGARATSPEAG GGGGALKASSAPAAAAGLLREAGSG GRERADWRRRQLRKVRSVELDQLPE QPLFLAASPPASSTSPSPEPADAAG SGTGFQPVAVPPPHGAASRGGAHLT ESVAAPDSGASSPAAAEPGEKRAPA AEPSPAAAPAGREMENKETLKGLHK MDDRPEERMIREKLKATCMPAWKHE WLERRNRRGPVVVKPIPVKGDGSEM NHLAAESPGEVQASAASPASKGRRS PSPGNSPSGRTVKSESPGVRRKRVS PVPFQSGRITPPRRAPSPDGFSPYS PEETNRRVNKVMRARLYLLQQIGPN SFLIGGDSPDNKYRVFIGPQNCSCA RGTFCIHLLFVMLRVFQLEPSDPML WRKTLKNFEVESLFQKYHSRRSSRI KAPSRNTIQKFVSRMSNSHTLSSSS TSTSSSENSIKDEEEQMCPICLLGM LDEESLTVCEDGCRNKLHHHCMSIW AEECRRNREPLICPLCRSKWRSHDF YSHELSSPVDSPSSLRAAQQQTVQQ QPLAGSRRNQESNFNLTHYGTQQIP PAYKDLAEPWIQVFGMELVGCLFSR NWNVREMALRRLSHDVSGALLLANG ESTGNSGGSSGSSPSGGATSGSSQT SISGDVVEACCSVLSMVCADPVYKV YVAALKTLRAMLVYTPCHSLAERIK LQRLLQPVVDTILVKCADANSRTSQ LSISTLLELCKGQAGELAVGREILK AGSIGIGGVDYVLNCILGNQTESNN WQELLGRLCLIDRLLLEFPAEFYPH IVSTDVSQAEPVEIRYKKLLSLLTF ALQSIDNSHSMVGKLSRRIYLSSAR MVTTVPHVFSKLLEMLSVSSSTHFT RMRRRLMAIADEVEIAEAIQLGVED TLDGQQDSFLQASVPNNYLETTENS SPECTVHLEKTGKGLCATKLSASSE DISERLASISVGPSSSTTTTTTTTE QPKPMVQTKGRPHSQCLNSSPLSHH SQLMFPALSTPSSSTPSVPAGTATD VSKHRLQGFIPCRIPSASPQTQRKF SLQFHRNCPENKDSDKLSPVFTQSR PLPSSNIHRPKPSRPTPGNTSKQGD PSKNSMTLDLNSSSKCDDSFGCSSN SSNAVIPSDETVFTPVEEKCRLDVN TELNSSIEDLLEASMPSSDTTVTFK SEVAVLSPEKAENDDTYKDDVNHNQ KCKEKMEAEEEEALAIAMAMSASQD ALPIVPQLQVENGEDIIIIQQDTPE TLPGHTKAKQPYREDTEWLKGQQIG LGAFSSCYQAQDVGTGTLMAVKQVT YVRNTSSEQEEVVEALREEIRMMSH LNHPNIIRMLGATCEKSNYNLFIEW MAGGSVAHLLSKYGAFKESVVINYT EQLLRGLSYLHENQIIHRDVKGANL LIDSTGQRLRIADFGAAARLASKGT GAGEFQGQLLGTIAFMAPEVLRGQQ YGRSCDVWSVGCAIIEMACAKPPWN AEKHSNHLALIFKIASATTAPSIPS HLSPGLRDVALRCLELQPQDRPPSR ELLKHPVFRTTWTRTRPLEQKLISE EDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
