Recombinant Human PLA2G5 Protein PLA2G5-44H

Recombinant Human PLA2G5 Protein


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Recombinant Human PLA2G5 Protein

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Cat.No. : PLA2G5-44H
Product Overview : Recombinant human PLA2G5 protein without tag was expressed in E. coli.
Description : This gene is a member of the secretory phospholipase A2 family. It is located in a tightly-linked cluster of secretory phospholipase A2 genes on chromosome 1. The encoded enzyme catalyzes the hydrolysis of membrane phospholipids to generate lysophospholipids and free fatty acids including arachidonic acid. It preferentially hydrolyzes linoleoyl-containing phosphatidylcholine substrates. Secretion of this enzyme is thought to induce inflammatory responses in neighboring cells. Alternatively spliced transcript variants have been found, but their full-length nature has not been determined.
Source : E. coli
Species : Human
Molecular Mass : 13.72 kDa
Purity : ≥95% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to release 1 μmol of TNB per minute under the following condition: 25 mM Tris-HCl, pH 7.5, containing 10 mM CaCl2, 100 mM KCl, 0.3 mM Triton X-100, 1 mg/mL BSA, 500 μM 1,2-bis(heptanoylthio) Glycerophosphocholine, and 0.5 mM DTNB at 37 centigrade.
Stability : ≥ 1 year
Storage : Store it at -80 centigrade
Storage Buffer : 50 mM Tris-HCl, pH 8.0, containing 100 mM sodium chloride, 50 mM calcium chloride, and 20% glycerol
SDS-PAGE Analysis of PLA2G5 :

Lane 1: MW Markers; Lane 2: sPLA2 Type V (4 μg); Lane 3: sPLA2 Type V (2 μg); Lane 4: sPLA2 Type V (1 μg). Representative gel image shown; actual purity may vary between each batch.

Activity Assay :

Gene Name : PLA2G5 phospholipase A2 group V [ Homo sapiens (human) ]
Official Symbol : PLA2G5
Synonyms : PLA2G5; phospholipase A2 group V; FRFB; GV-PLA2; PLA2-10; hVPLA(2); phospholipase A2 group V; calcium-dependent phospholipase A2; Ca2+-dependent phospholipase A2; phosphatidylcholine 2-acylhydrolase 5
Gene ID : 5322
mRNA Refseq : NM_000929
Protein Refseq : NP_000920
MIM : 601192
UniProt ID : P39877

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