Recombinant Human ROCK2, GST-His

Cat.No. : ROCK2-712H
Product Overview : Recombinant Human ROCK2, Amino acids P6-S553, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells. MW = 92,279 Da. .
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Cat. No. : ROCK2-712H
Description : ROCK2 is a ubiquitously expressed serine/threonine kinase localized in the nucleus that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. ROCK2 is an immediate downstream target of the small GTPase RhoA.
Source : Baculovirus infected Sf9 cells.
Purification : One-step affinity purification using GSH-agarose.
Product Identity : ROCK2 was confirmed as human ROCK2 by mass spec troscopy LC-ESI-MS/MS (Protagen AG, Germany).
Storage Buffer : 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
Concentration : 0.356 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
Specific Activity : 27 pmol/μg×min.
Storage : -80°C. Avoid repeated freeze-thaw cycles!
Protein length : P6-S553
Gene Name : ROCK2 Rho-associated, coiled-coil containing protein kinase 2 [ Homo sapiens ]
Synonyms : ROCK2; Rho-associated, coiled-coil containing protein kinase 2; KIAA0619; EC 2.7.11.1
Gene ID : 9475
mRNA Refseq : NM_004850
Protein Refseq : NP_004841
MIM : 604002
UniProt ID : O75116
Chromosome Location : 2p24
Pathway : Axon guidance; Chemokine signaling pathway; Focal adhesion; Leukocyte transendothelial migration; Pathogenic Escherichia coli infection; Regulation of actin cytoskeleton; TGF-beta signaling pathway; Vascular smooth muscle contraction; Wnt signaling pathway; Signaling by GPCR; Axon guidance
Function : ATP binding; GTP-Rho binding; identical protein binding; magnesium ion binding; nucleotide binding; protein serine/threonine kinase activity; zinc ion binding; transferase activity

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08/19/2021

    Swift and hassle-free delivery ensured our experiments stayed on track.

    01/13/2020

      Efficient customer service provided valuable insights for our decision-making.

      07/06/2019

        Reliable and precise—this recombined protein never disappoints.

        Q&As (5)

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        Discuss the role of ROCK2 in smooth muscle contraction and vascular tone. 03/24/2022

        ROCK2 is a key regulator of smooth muscle contraction, influencing vascular tone by phosphorylating myosin light chain and promoting actin-myosin interaction.

        Describe the molecular crosstalk between ROCK2 and other signaling pathways. 08/07/2021

        ROCK2 interacts with various signaling pathways, including Wnt, PI3K/Akt, and MAPK pathways, forming a network that regulates cell survival, proliferation, and differentiation.

        How does ROCK2 contribute to the regulation of cell cycle progression? 12/04/2020

        ROCK2 can influence the cell cycle by regulating cyclin-dependent kinase activity and promoting the phosphorylation of proteins involved in cell cycle checkpoints.

        What are the implications of ROCK2 in cell migration and invasion? 03/18/2019

        ROCK2 is involved in the regulation of cell migration and invasion by controlling actin dynamics and focal adhesion turnover, influencing processes like metastasis in cancer.

        Discuss the role of ROCK2 in the regulation of the actin cytoskeleton. 06/11/2018

        ROCK2 phosphorylates various downstream targets, such as myosin light chain, leading to actin-myosin contraction and the reorganization of the actin cytoskeleton.

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