Recombinant Human ROCK2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: ROCK2-1165H
• Product Overview: ROCK2 MS Standard C13 and N15-labeled recombinant protein (NP_004841) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 160.7 kDa
• AA Sequence: MSRPPPTGKMPGAPETAPGDGAGAS RQRKLEALIRDPRSPINVESLLDGL NSLVLDLDFPALRKNKNIDNFLNRY EKIVKKIRGLQMKAEDYDVVKVIGR GAFGEVQLVRHKASQKVYAMKLLSK FEMIKRSDSAFFWEERDIMAFANSP WVVQLFYAFQDDRYLYMVMEYMPGG DLVNLMSNYDVPEKWAKFYTAEVVL ALDAIHSMGLIHRDVKPDNMLLDKH GHLKLADFGTCMKMDETGMVHCDTA VGTPDYISPEVLKSQGGDGFYGREC DWWSVGVFLYEMLVGDTPFYADSLV GTYSKIMDHKNSLCFPEDAEISKHA KNLICAFLTDREVRLGRNGVEEIRQ HPFFKNDQWHWDNIRETAAPVVPEL SSDIDSSNFDDIEDDKGDVETFPIP KAFVGNQLPFIGFTYYRENLLLSDS PSCRETDSIQSRKNEESQEIQKKLY TLEEHLSNEMQAKEELEQKCKSVNT RLEKTAKELEEEITLRKSVESALRQ LEREKALLQHKNAEYQRKADHEADK KRNLENDVNSLKDQLEDLKKRNQNS QISTEKVNQLQRQLDETNALLRTES DTAARLRKTQAESSKQIQQLESNNR DLQDKNCLLETAKLKLEKEFINLQS ALESERRDRTHGSEIINDLQGRICG LEEDLKNGKILLAKVELEKRQLQER FTDLEKEKSNMEIDMTYQLKVIQQS LEQEEAEHKATKARLADKNKIYESI EEAKSEAMKEMEKKLLEERTLKQKV ENLLLEAEKRCSLLDCDLKQSQQKI NELLKQKDVLNEDVRNLTLKIEQET QKRCLTQNDLKMQTQQVNTLKMSEK QLKQENNHLMEMKMNLEKQNAELRK ERQDADGQMKELQDQLEAEQYFSTL YKTQVRELKEECEEKTKLGKELQQK KQELQDERDSLAAQLEITLTKADSE QLARSIAEEQYSDLEKEKIMKELEI KEMMARHKQELTEKDATIASLEETN RTLTSDVANLANEKEELNNKLKDVQ EQLSRLKDEEISAAAIKAQFEKQLL TERTLKTQAVNKLAEIMNRKEPVKR GNDTDVRRKEKENRKLHMELKSERE KLTQQMIKYQKELNEMQAQIAEESQ IRIELQMTLDSKDSDIEQLRSQLQA LHIGLDSSSIGSGPGDAEADDGFPE SRLEGWLSLPVRNNTKKFGWVKKYV IVSSKKILFYDSEQDKEQSNPYMVL DIDKLFHVRPVTQTDVYRADAKEIP RIFQILYANEGESKKEQEFPVEPVG EKSNYICHKGHEFIPTLYHFPTNCE ACMKPLWHMFKPPPALECRRCHIKC HKDHMDKKEEIIAPCKVYYDISTAK NLLLLANSTEEQQKWVSRLVKKIPK KPPAPDPFARSSPRTSMKIQQNQSI RRPSRQLAPNKPSTRTRPLEQKLIS EEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: ROCK2 Rho associated coiled-coil containing protein kinase 2 [ Homo sapiens (human) ]
• Official Symbol: ROCK2
• Synonyms: ROCK2; Rho-associated, coiled-coil containing protein kinase 2; rho-associated protein kinase 2; p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase II; ROCK-II; KIAA0619
• Gene ID: 9475
• mRNA Refseq: NM_004850
• Protein Refseq: NP_004841
• MIM: 604002
• UniProt ID: O75116

Reviews

08/19/2021

Swift and hassle-free delivery ensured our experiments stayed on track.

01/13/2020

Efficient customer service provided valuable insights for our decision-making.

07/06/2019

Reliable and precise—this recombined protein never disappoints.

Q&As

Discuss the role of ROCK2 in smooth muscle contraction and vascular tone.

ROCK2 is a key regulator of smooth muscle contraction, influencing vascular tone by phosphorylating myosin light chain and promoting actin-myosin interaction.

Describe the molecular crosstalk between ROCK2 and other signaling pathways.

ROCK2 interacts with various signaling pathways, including Wnt, PI3K/Akt, and MAPK pathways, forming a network that regulates cell survival, proliferation, and differentiation.

How does ROCK2 contribute to the regulation of cell cycle progression?

ROCK2 can influence the cell cycle by regulating cyclin-dependent kinase activity and promoting the phosphorylation of proteins involved in cell cycle checkpoints.

What are the implications of ROCK2 in cell migration and invasion?

ROCK2 is involved in the regulation of cell migration and invasion by controlling actin dynamics and focal adhesion turnover, influencing processes like metastasis in cancer.

Discuss the role of ROCK2 in the regulation of the actin cytoskeleton.

ROCK2 phosphorylates various downstream targets, such as myosin light chain, leading to actin-myosin contraction and the reorganization of the actin cytoskeleton.