Recombinant Human SET nuclear oncogene, His-tagged

• Cat.No.: SET-237H
• Product Overview: SET, 1-290aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-290 a.a.
• Description: SET inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene. Recombinant human SET protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT.
• Molecular Mass: 35.9 kDa (313aa), confirmed by MALDI-TOF(Molecular size on SDS-PAGE will appear higher)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAPKRQS PLPPQKKKPR PPPALGPEET SASAGLPKKG EKEQQEAIEH IDEVQNEIDR LNEQASEEIL KVEQKYNKLR QPFFQKRSEL IAKIPNFWVT TFVNHPQVSA LLGEEDEEAL HYLTRVEVTE FEDIKSGYRI DFYFDENPYF ENKVLSKEFH LNESGDPSSK STEIKWKSGK DLTKRSSQTQ NKASRKRQHE EPESFFTWFT DHSDAGADEL GEVIKDDIWP NPLQYYLVPD MDDEEGEGEE DDDDDEEEEG LEDIDEEGDE DEGEEDEDDD EGEEGEEDEG EDD
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: SET SET nuclear oncogene [ Homo sapiens ]
• Official Symbol: SET
• Synonyms: SET; SET nuclear oncogene; SET translocation (myeloid leukemia associated); protein SET; 2PP2A; IPP2A2; PHAPII; protein phosphatase type 2A inhibitor; Template Activating Factor I; chromatin remodelling factor; HLA-DR-associated protein II; phosphatase 2A inhibitor I2PP2A; inhibitor-2 of protein phosphatase-2A; inhibitor of granzyme A-activated DNase; SET translocation (myeloid leukemia-associated); Template-Activating Factor-I, chromatin remodelling factor; IGAAD; TAF-I; I2PP2A; TAF-IBETA
• Gene ID: 6418
• mRNA Refseq: NM_001122821
• Protein Refseq: NP_001116293
• MIM: 600960
• UniProt ID: Q01105
• Chromosome Location: 9q34
• Pathway: Gene Expression, organism-specific biosystem; Regulation of mRNA Stability by Proteins that Bind AU-rich Elements, organism-specific biosystem; Stabilization of mRNA by HuR, organism-specific biosystem
• Function: histone binding; protein binding; protein phosphatase inhibitor activity; protein phosphatase type 2A regulator activity