Recombinant Human SPN Protein, His-tagged

• Cat.No.: SPN-11043H
• Product Overview: Human CD43, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 20 - Arg 253 (Accession # P16150-1 ). This protein carries a polyhistidine tag at the C-terminus. Predicted N-terminus: Ser 20

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 20-253 aa
• Description: CD43 or leukosialin or sialophorin, is predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. In hematopathology, CD43 is often considered as a T-cell associated marker, because it is expressed by over 95% of thymocytes and peripheral blood T cells. CD43 positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN).
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Molecular Mass: The protein has a calculated MW of 25.3 kDa. The protein migrates as 45-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU/μg by the LAL method/rFC method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: SPN sialophorin [ Homo sapiens ]
• Official Symbol: SPN
• Synonyms: SPN; sialophorin; sialophorin (gpL115, leukosialin, CD43); leukosialin; CD43; GPL115; LSN; GALGP; galactoglycoprotein; leukocyte sialoglycoprotein; sialophorin (leukosialin, CD43)
• Gene ID: 6693
• mRNA Refseq: NM_001030288
• Protein Refseq: NP_001025459
• MIM: 182160
• UniProt ID: P16150