Recombinant Human TDGF1 cell lysate

• Cat.No.: TDGF1-2432HCL
• Product Overview: Human TDGF1 / CRGF derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: TDGF1 teratocarcinoma-derived growth factor 1 [ Homo sapiens ]
• Official Symbol: TDGF1
• Synonyms: TDGF1; teratocarcinoma-derived growth factor 1; CR; CRIPTO; Cripto 1; cripto-1 growth factor; epidermal growth factor-like cripto protein CR1; CRGF
• Gene ID: 6997
• mRNA Refseq: NM_001174136
• Protein Refseq: NP_001167607
• UniProt ID: P13385
• Chromosome Location: 3p21.31
• Pathway: Developmental Biology, organism-specific biosystem; Glypican 1 network, organism-specific biosystem; Regulation of Signaling by NODAL, organism-specific biosystem; Signaling by NODAL, organism-specific biosystem
• Function: growth factor activity; protein binding; receptor binding

Reviews

01/13/2020

the manufacturer can assist in sourcing complementary reagents and tools, such as specific antibodies or detection systems, to complement the use of TDGF1 protein in trials.

03/26/2018

manufacturers often have a wealth of knowledge and expertise in the field of TDGF1 protein research.

08/29/2017

They may offer educational resources such as technical guides, protocols, and application notes, which can aid researchers in understanding the principles, techniques, and potential applications of TDGF1 protein in their studies.

Q&As

Is TDGF1 associated with any genetic disorders?

Mutations in the TDGF1 gene have been linked to certain congenital disorders. Understanding these genetic associations can provide insights into the underlying mechanisms of these conditions.

How does TDGF1 influence neurodevelopment?

TDGF1 has been found to play a role in neural development. It is involved in the specification of neural cell fate and the formation of the neural tube during embryogenesis.

Can TDGF1 be a target for drug development?

Yes, efforts are underway to develop drugs that modulate TDGF1 activity. Targeting TDGF1 could potentially lead to novel therapies for conditions related to cell growth and differentiation.

In what ways does TDGF1 contribute to stem cell biology?

TDGF1 is involved in the maintenance of pluripotency in embryonic stem cells. Understanding its role could enhance our ability to control and manipulate stem cells for therapeutic purposes.

How can TDGF1 be utilized in cancer diagnostics?

Researchers are exploring the use of TDGF1 as a biomarker for certain cancers. Detection of elevated TDGF1 levels in patient samples may aid in early cancer diagnosis.