Active Recombinant Human TGFB1 Protein, Pre-aliquoted

• Cat.No.: TGFB1-17H
• Product Overview: Recombinant Human TGFB1 Protein, Ala279 - Ser390, was expressed in CHO.

Specification

• Description: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.
• Source: CHO
• Species: Human
• Bio-activity: Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. The ED50 for this effect is 0.04-0.2 ng/mL.
• Molecular Mass: Predicted Molecular Mass:12.8 kDa (monomer)
  SDS-PAGE: 12 kDa, reducing conditions, 24 kDa, non-reducing conditions
• Protein length: Ala279-Ser390
• N-terminal Sequence Analysis: Ala279
• Endotoxin: < 0.01 EU/μg of the protein by the LAL method.
• Purity: > 97% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature as supplied.
  • 12 months from date of receipt at 2-8 centigrade as supplied.
  • 1 month at 2-8 centigrade under sterile conditions after reconstitution.
  • 3 months at -20 to -80 centigrade under sterile conditions after reconstitution.
• Reconstitution: For a stock solution, reconstitute at 20 μg/mL in sterile 4 mM HCl, or simply roll Pre-aliquoted directly into cell culture medium for immediate use.
  Note: Purified recombinant human TGF-beta 1 is an extremely hydrophobic protein that adheres strongly to surfaces.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: TGFB1 transforming growth factor beta 1 [ Homo sapiens (human) ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor beta 1; CED; LAP; DPD1; TGFB; IBDIMDE; TGFbeta; TGF-beta1; transforming growth factor beta-1 proprotein; TGF-beta-1; latency-associated peptide; prepro-transforming growth factor beta-1; transforming growth factor beta1
• Gene ID: 7040
• mRNA Refseq: NM_000660
• Protein Refseq: NP_000651
• MIM: 190180
• UniProt ID: P01137

Reviews

11/16/2021

The impressive performance of the TGFB1 protein both in ELISA assays and protein electron microscopy structure analysis makes it a highly versatile and recommended choice for scientists across various disciplines.

03/01/2020

Its reliability and efficacy in generating high-quality data make it an indispensable tool in the quest for unraveling the mysteries of protein structure and function.

07/16/2018

The TGFB1 protein's excellent performance in ELISA ensures accurate and reproducible results, enhancing the reliability of experimental data.

Q&As

How is TGFB1 involved in bone remodeling?

TGFB1 stimulates the activity of cells involved in bone formation (osteoblasts) and plays a role in regulating bone resorption by influencing osteoclast function.

Are there any side effects associated with therapies targeting TGFB1?

Potential side effects of anti-TGFB1 therapies may include immune system suppression, gastrointestinal issues, and skin reactions.

Can TGFB1 be used as a biomarker for certain diseases?

TGFB1 levels have been studied as potential biomarkers for diseases like cancer and fibrosis, aiding in diagnosis and monitoring disease progression.

In what ways does TGFB1 influence wound healing?

TGFB1 plays a critical role in wound healing by stimulating the formation of new blood vessels, promoting the production of extracellular matrix, and aiding in the migration of cells to the wound site.

How does TGFB1 contribute to fibrosis?

TGFB1 can induce the production of excessive extracellular matrix proteins, leading to tissue scarring and fibrosis in organs such as the liver, lung, and kidneys.