"THPO" Related Products

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Recombinant Human THPO Protein, His-tagged

Cat.No.: THPO-216H
Product Overview: Recombinant mature form of human THPO (NP_000451.1) (Ser 22-Gly 353) was fused with a polyhistidine tag at the N-terminus.
Source: HEK293
Species: Human
Tag: His
Predicted N Terminal: Ser 22
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 3-15 ng/ml.
Molecular Mass: The recombinant human THPO consists of 348 amino acids with the predicted molecular mass of 37.6 kDa. As a result of glycosylation, rhTHPO migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: THPO thrombopoietin [ Homo sapiens ]
Official Symbol: THPO
Synonyms: ML; TPO; MGDF; MKCSF; MPLLG; THCYT1; MPL ligand; c-mpl ligand; megakaryocyte colony-stimulating factor; megakaryocyte growth and development factor; megakaryocyte stimulating factor; myeloproliferative leukemia virus oncogene ligand; prepro-thrombopoietin
Gene ID: 7066
mRNA Refseq: NM_000460
Protein Refseq: NP_000451
MIM: 600044
UniProt ID: P40225
Chromosome Location: 3q27
Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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