Recombinant Human Thrombopoietin

• Cat.No.: THPO-176H

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Non
• Description: Thrombopoietin (leukemia virus oncogene ligand, megakaryocyte growth and development factor), also known as THPO is a lineage-dominant hematopoietic cytokine. TPO is a glycoprotein hormone constitutively produced mainly by the liver and kidney and is the primary physiological regulator megakaryopoiesis and platelet production.
• Molecular Weight: The predicted molecular weight of Recombinant Human TPO is Mr 35 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 80-90 kDa.
• State Of Matter: Lyophilized.
• Biological Activity: The biological activity of Human Thrombopoietin is determined by the dose-dependent stimulation of the proliferation of human MO7e cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.
• Purity: >90% by SDS Page and analyzed by silver stain.
• Endotoxin: <1.0 EU/µg as determined by the LAL method.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Gene Information

• Gene Name: THPO thrombopoietin [ Homo sapiens ]
• Synonyms: thrombopoietin nirs variant 1; megakaryocyte stimulating factor; MKCSF; c-mpl ligand; megakaryocyte colony-stimulating factor; MGDF; megakaryocyte growth and development factor; MPL ligand; myeloproliferative leukemia virus oncogene ligand; C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGC163194; Myeloproliferative leukemia virus oncogene ligand; Thrombopoietin; ML; TPO; MPLLG; THPO
• Gene ID: 7066
• mRNA Refseq: NM_000460
• Protein Refseq: NP_000451
• MIM: 600044
• UniProt ID: P40225
• Chromosome Location: 3q27
• Pathway: Hematopoietic cell lineage
• Function: cytokine activity; growth factor activity; hormone activity