Recombinant Human WDR77 293 Cell Lysate

• Cat.No.: WDR77-333HCL

Specification

• Description: Antigen standard for WD repeat domain 77 (WDR77) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: WDR77 WD repeat domain 77 [ Homo sapiens ]
• Official Symbol: WDR77
• Synonyms: WDR77; WD repeat domain 77; methylosome protein 50; MEP50; androgen receptor cofactor p44; WD repeat-containing protein 77; p44; MEP-50; HKMT1069; Nbla10071; p44/Mep50; MGC2722; RP11-552M11.3
• Gene ID: 79084
• mRNA Refseq: NM_024102
• Protein Refseq: NP_077007
• MIM: 611734
• UniProt ID: Q9BQA1
• Chromosome Location: 1p13.2
• Pathway: Gene Expression, organism-specific biosystem; Metabolism of non-coding RNA, organism-specific biosystem; Signaling events mediated by HDAC Class I, organism-specific biosystem; snRNP Assembly, organism-specific biosystem

Reviews

06/09/2019

Crucial for decision-making. Accurate and efficient protein analysis.

10/04/2018

Exceeds research standards. Trusted for accurate data.

03/29/2017

Dependable partner in our experiments. Consistently meets expectations.

Q&As

What role does WDR77 play in cancer development and progression?

WDR77 can influence cancer progression by regulating cell growth and division.

What is the impact of WDR77 dysregulation in hormonal cancers, such as prostate cancer?

WDR77 dysregulation is particularly significant in hormonal cancers, impacting androgen receptor signaling.

How does WDR77 contribute to the regulation of gene expression and protein synthesis?

It plays a role in gene expression by participating in the formation of the spliceosome and ribosome assembly.

What potential therapeutic strategies could target WDR77 in cancer treatment?

Targeting WDR77 in therapy could provide new avenues for treating cancers, especially hormone-driven cancers like prostate cancer.

How does WDR77 interact with other proteins and signaling pathways in the cell?

WDR77 interacts with molecular chaperones and is involved in multiple signaling pathways, influencing cell cycle and apoptosis.

What is the primary function of WDR77 in cellular processes?

WDR77 is involved in various cellular functions, including RNA processing and ribosome biogenesis.

How do genetic alterations in WDR77 affect its function and contribute to disease development?

Mutations in WDR77 can disrupt its normal functions, potentially leading to uncontrolled cell proliferation and cancer.