Optional Service: Optional requirements on this protein
Product Overview :
Alexa Fluor 555 conjugated recombinant Mouse CD28 precursor extracellular domain (Met 1-Lys 149) (NP_031668.3), fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus, was produced in Human Cell.
Molecular Mass :
The recombinant mouse CD28/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 378 amino acids and has a predicted molecular mass of 43 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD28/Fc monomer is approximately 55-60 kDa due to glycosylation.
Protein length :
< 1.0 EU/ μg of the protein as determined by the LAL method.
Disulfide-linked homodimer Labeled with Alexa Fluor 555 via amines With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
Samples are stable for up to 12 months from date of receipt at -70 centigrade.
Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Storage Buffer :
Lyophilized from sterile PBS, pH 7.4
It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.