| Species : |
Mouse |
| Source : |
E.coli |
| Tag : |
GST&His |
| Protein Length : |
25-96 aa |
| Form : |
Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| Bio-activity : |
Neutrophil-activating protein 3 (NAP3) also known as chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family. NAP3 is expressed by macrophages, neutrophils and epithelial cells,and has neutrophil chemoattractant activity. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of NAP3 on THP-1 the human monocytic cell line. Briefly, THP-1 cells were seeded into the upper chambers (150 ul cell suspension,10^6 cells/ml in RPMI 1640 with FBS free) and different concentrations of recombinant mouse NAP3 diluted with serum free RPMI 1640 was added in lower chamber with a polycarbonate filter (8 um pore size) used to separate the two compartments. After incubation at 37°C with 5% CO2 for 1h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification(×400) randomly (five fields for each filter). Result shows recombinant mouse NAP3 is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (×100) were shown. The optimum chemotaxis of NAP3 occurs at 0.001 ng/ml. |
| Molecular Mass : |
37.9kDa |
| Endotoxin : |
<1.0EU per 1μg (determined by the LAL method). |
| Purity : |
> 90% as determined by SDS-PAGE. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
