Active Recombinant Mouse Egfr protein, His-tagged, Low Endotoxin

• Cat.No.: Egfr-5224M
• Product Overview: Recombinant Mouse Egfr protein(Q01279)(25-647 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 25-647 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Mouse EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse EGF, hFc Tag with an affinity constant of 3.98 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 70.40 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Egfr epidermal growth factor receptor [ Mus musculus ]
• Official Symbol: Egfr
• Synonyms: EGFR; epidermal growth factor receptor; waved 2; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; Wa5; wa2; Erbb; Errp; wa-2; AI552599; 9030024J15Rik
• Gene ID: 13649
• mRNA Refseq: NM_007912
• Protein Refseq: NP_031938