Recombinant Mouse F12 Protein, His-tagged

• Cat.No.: F12-909M
• Product Overview: Recombinant Mouse F12 Protein (Ala20-Ser597) with His tag was expressed in 293F.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Ala20-Ser597
• Description: This gene encodes a glycoprotein coagulation factor that plays an important role in the intrinsic pathway of blood coagulation and hemostasis. The encoded protein is an inactive zymogen that is autoactivated upon contact with negatively charged surfaces or misfolded protein aggregates. Mice lacking the encoded protein have a severe defect in forming stable fibrin clots.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 65.3 kDa; Accurate Molecular Mass: 110 kDa
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: F12 coagulation factor XII (Hageman factor) [ Mus musculus (house mouse) ]
• Official Symbol: F12
• Synonyms: F12; coagulation factor XII (Hageman factor); coagulation factor XII; factor XII; hageman factor; HAF; FXII
• Gene ID: 58992
• mRNA Refseq: NM_021489
• Protein Refseq: NP_067464
• UniProt ID: Q80YC5