Recombinant Mouse FGF18 protein(Met1-Gly207), His-tagged

• Cat.No.: Fgf18-491M
• Product Overview: Recombinant Mouse FGF18 (NP_032031.1) (Met 1-Gly 207) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-207 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse FGF18 at 10 μg/mL (100 μl/well) can bind Rat FGFR4, The EC50 of Rat FGFR4 is 0.44 μg/mL.
• Molecular Mass: The secreted recombinant mouse FGF18 comprises 191 amino acids and has a predicted molecular mass of 22.5 kDa. As a result of glycosylation, it migrates as an approximately 30-35 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fgf18 fibroblast growth factor 18 [ Mus musculus ]
• Official Symbol: FGF18
• Synonyms: FGF18; fibroblast growth factor 18; zFGF5; FGF-18; D130055P09Rik
• Gene ID: 14172
• mRNA Refseq: NM_008005
• Protein Refseq: NP_032031