Recombinant Mouse Fgf21 protein
Cat.No. : | Fgf21-563M |
Product Overview : | Recombinant Mouse Fgf21 protein was expressed in Escherichia coli. |
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Description : | Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. FGF-21, in the presence of betaKlotho as a protein cofactor, signals through the FGFR 1c and 4 receptors. Recombinant murine FGF-21 contains 182 amino acids residues and show limited binding to heparin. In addition, Murine FGF-21 respectively shows 81 % and 92 % a.a. identity to human and rat FGF-21, and it show activity on human and rat cells. |
Source : | E.coli |
Species : | Mouse |
Form : | Lyophilized from a 0.2μm filtered concentrated solution in 3 × PBS, pH 7.4. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 × 10³ IU/mg in the presence of 5 µg/ml of rMuKlotho-β and 10 μg/ml of heparin. |
Molecular Mass : | Approximately 19.9 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids. |
Protein length : | 182 |
AA Sequence : | AYPIPDSSPLLQFGGQVRQRYLYTD DDQDTEAHLEIREDGTVVGAAHRSP ESLLELKALKPGVIQILGVKASRFL CQQPDGALYGSPHFDPEACSFRELL LEDGYNVYQSEAHGLPLRLPQKDSP NQDATSWGPVRFLPMPGLLHEPQDQ AGFLPPEPPDVGSSDPLSMVEPLQG RSPSYAS |
Endotoxin : | Less than 1 EU/µg of rMuFGF-21 as determined by LAL method. |
Purity : | >97% by SDS-PAGE and HPLC analysis. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Publication : |
Alcoholic fatty liver is enhanced in CYP2A5 knockout mice: The role of the PPARα-FGF21 axis. (2017)
Deficiency of fibroblast growth factor 21 aggravates obesity-induced atrophic responses in skeletal muscle (2019)
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Gene Name : | Fgf21 |
Official Symbol : | Fgf21 |
Synonyms : | FGF21; fibroblast growth factor 21; FGF-21; |
Gene ID : | 56636 |
mRNA Refseq : | NM_020013 |
Protein Refseq : | NP_064397 |
UniProt ID : | Q9JJN1 |
Products Types
◆ Recombinant Protein | ||
FGF21-85H | Active Recombinant Human FGF21 Protein | +Inquiry |
FGF21-415F | Active Recombinant Human FGF21 Protein (182 aa) | +Inquiry |
FGF21-120F | Active Recombinant Human FGF21 Protein (182 aa) | +Inquiry |
FGF21-287F | Active Recombinant Human FGF21 Protein, His-tagged | +Inquiry |
Fgf21-1490R | Recombinant Rat Fgf21 Protein, His-tagged | +Inquiry |
◆ Lysates | ||
FGF21-1890MCL | Recombinant Mouse FGF21 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionFGF21 expression in adipose tissue is regulated by various factors, including peroxisome proliferator-activated receptor gamma (PPARγ) activation.
FGF21 influences lipid metabolism by promoting the utilization of fatty acids for energy, contributing to the regulation of lipid homeostasis.
FGF21 levels are being explored as potential biomarkers for metabolic health, reflecting conditions such as insulin resistance and metabolic syndrome.
Yes, FGF21 plays a role in improving insulin sensitivity, making it a potential target for interventions in insulin resistance-associated conditions.
Yes, FGF21 influences energy expenditure by promoting thermogenesis in brown adipose tissue, contributing to overall metabolic regulation.
Gender-specific differences in FGF21 levels have been reported, with some studies suggesting higher circulating FGF21 concentrations in females.
FGF21 activates signaling pathways such as the AMP-activated protein kinase (AMPK) pathway, which contributes to its metabolic effects.
Customer Reviews (3)
Write a reviewThe manufacturer's history of producing high-quality products contributed to our trust in this protein.
Collaboration with other research groups was made easier by the product's standardized use in the scientific community.
The product's role in promoting collaboration and teamwork positively impacted the quality of our research.
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