Recombinant Mouse Grk5 Protein, MYC/DDK-tagged

• Cat.No.: Grk5-1051M
• Product Overview: Purified recombinant protein of full-length mouse G protein-coupled receptor kinase 5 (Grk5), with C-terminal MYC/DDK tag, was expressed in HEK293T cells.

Specification

• Description: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).
• Source: HEK293T
• Species: Mouse
• Tag: MYC/DDK
• Molecular Mass: 67.7 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 µg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Grk5 G protein-coupled receptor kinase 5 [ Mus musculus (house mouse) ]
• Official Symbol: Grk5
• Synonyms: Grk5; G protein-coupled receptor kinase 5; Gprk5; G protein-coupled receptor kinase 5; g protein-coupled receptor kinase GRK5; EC 2.7.11.16
• Gene ID: 14773
• mRNA Refseq: NM_018869
• Protein Refseq: NP_061357
• UniProt ID: Q8VEB1