Expand Expand| Cat.No. : | IL17A-381M |
| Product Overview : | Recombinant Mouse IL17A Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Ala 158 (Accession # Q62386-1). Predicted N-terminus: His. This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 15.8 kDa. The protein migrates as 18 kDa, 20-23 kDa and 26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Source : | HEK293 |
| Species : | Mouse |
| Tag : | His |
| Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : | Recombinant Mouse IL17A Protein, His-tagged at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag with a linear range of 8-128 ng/mL (QC tested). Recombinant Mouse IL17A Protein, His-tagged stimulates secretion of IL-6 by NIH-3T3 cells. The ED50 for this effect is 2.132-5.005 ng/ml. |
| Molecular Mass : | 15.8 kDa |
| Protein length : | Ala 26 - Ala 158 |
| Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
| Purity : | >90% as determined by SDS-PAGE. |
| Notes : | Please avoid repeated freeze-thaw cycles. |
| Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
| Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
| Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
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| Gene Name : | Il17a interleukin 17A [ Mus musculus ] |
| Official Symbol : | IL17A |
| Synonyms : | IL17A; interleukin 17A; interleukin-17A; Il17; Ctla8; IL-17; Ctla-8; IL-17A; |
| Gene ID : | 16171 |
| mRNA Refseq : | NM_010552 |
| Protein Refseq : | NP_034682 |
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