| Cat.No. : |
IL17A-381M |
| Product Overview : |
Recombinant Mouse IL17A Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Ala 158 (Accession # Q62386-1). Predicted N-terminus: His. This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 15.8 kDa. The protein migrates as 18 kDa, 20-23 kDa and 26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Source : |
HEK293 |
| Species : |
Mouse |
| Tag : |
His |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Recombinant Mouse IL17A Protein, His-tagged at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag with a linear range of 8-128 ng/mL (QC tested).
Recombinant Mouse IL17A Protein, His-tagged stimulates secretion of IL-6 by NIH-3T3 cells. The ED50 for this effect is 2.132-5.005 ng/ml. |
| Molecular Mass : |
15.8 kDa |
| Protein length : |
Ala 26 - Ala 158 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>90% as determined by SDS-PAGE. |
| Notes : |
Please avoid repeated freeze-thaw cycles. |
| Stability : |
No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
| Figure : |
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