Recombinant Mouse IL17A Protein, His-tagged

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Recombinant Mouse IL17A Protein, His-tagged

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Cat.No. : IL17A-381M
Product Overview : Recombinant Mouse IL17A Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Ala 158 (Accession # Q62386-1). Predicted N-terminus: His. This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 15.8 kDa. The protein migrates as 18 kDa, 20-23 kDa and 26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Source : HEK293
Species : Mouse
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Recombinant Mouse IL17A Protein, His-tagged at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag with a linear range of 8-128 ng/mL (QC tested).
Recombinant Mouse IL17A Protein, His-tagged stimulates secretion of IL-6 by NIH-3T3 cells. The ED50 for this effect is 2.132-5.005 ng/ml.
Molecular Mass : 15.8 kDa
Protein length : Ala 26 - Ala 158
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Figure :

Gene Name : Il17a interleukin 17A [ Mus musculus ]
Official Symbol : IL17A
Synonyms : IL17A; interleukin 17A; interleukin-17A; Il17; Ctla8; IL-17; Ctla-8; IL-17A;
Gene ID : 16171
mRNA Refseq : NM_010552
Protein Refseq : NP_034682

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