Recombinant Mouse IL18BP protein, His-tagged

• Cat.No.: IL18BP-767M
• Product Overview: Recombinant Mouse IL18BP protein(Q9Z0M9)(Thr29-Ala193), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Thr29-Ala193
• Tag: C-His
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Mouse IL-18BP, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated IL-18, No Tag with the EC50 of 23.8ng/ml determined by ELISA (QC Test).
• Molecular Mass: The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  Production of IL-18 Binding Protein by Radiosensitive and Radioresistant Cells in CpG-Induced Macrophage Activation Syndrome (2020)
  Detection of Free Bioactive IL-18 and IL-18BP in Inflammatory Disorders (2023)
  Interleukin-18 as a Therapeutic Target in Western-diet Induced Cardiomyopathy (2017)
  Development of anti-murine IL-18 binding protein antibodies to stimulate IL-18 bioactivity (2025)

Gene Information

• Gene Name: Il18bp interleukin 18 binding protein [ Mus musculus ]
• Official Symbol: IL18BP
• Synonyms: IL18BP; interleukin 18 binding protein; interleukin-18-binding protein; interferon gamma inducing factor binding protein; interferon gamma-inducing factor-binding protein; MC54L; Igifbp; IL-18BP
• Gene ID: 16068
• mRNA Refseq: NM_010531
• Protein Refseq: NP_034661