Recombinant Mouse IL28B protein IL28B-65M

Recombinant Mouse IL28B protein

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Recombinant Mouse IL28B protein

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Cat.No. : IL28B-65M Optional Service: Optional requirements on this protein
Product Overview : Recombinant Mouse IL28B protein was expressed in Escherichia coli.
Description : This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA).
Source : E.coli
Species : Mouse
Form : Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 30 ng/ml, corresponding to a specific activity of > 3.3 × 10⁴ IU/mg.
Molecular Mass : Approximately 19.6 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.
Protein length : 174
AA Sequence : DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKGAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENINDS ALTTILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFQLL LRDLKCVASG DQCV
Endotoxin : Less than 1 EU/µg of rMuIFN-λ3/IL-28B as determined by LAL method.
Purity : >98% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
SDS-PAGE of IL28B-65M :

Gene Name : IL28B
Official Symbol : IL28B
Synonyms : IL28B; interleukin 28B; interleukin-28B; IFN-lambda-3; interleukin 28; interferon alpha; interferon lambda-3; Il28; IFL-1; IL-28B; INF-alpha; INF-lambda;
Gene ID : 338374
mRNA Refseq : NM_177396
Protein Refseq : NP_796370
UniProt ID : Q8CGK6
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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