Recombinant Mouse Il2ra Protein, His-tagged, Alexa Fluor 647 conjugated

• Cat.No.: Il2ra-4021MAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant Mouse Il2ra (Glu22-Lys236) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.
• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 26.6 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Glu22-Lys236
• N-terminal Sequence Analysis: Glu 22
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Il2ra
• Official Symbol: Il2ra
• Synonyms: IL2RA; interleukin 2 receptor, alpha chain; interleukin-2 receptor subunit alpha; IL2-RA; IL-2-RA; p55 chain; IL-2R alpha chain; IL-2R subunit alpha; IL-2 receptor subunit alpha; CD25; Il2r; Ly-43; MGC130561
• Gene ID: 16184
• mRNA Refseq: NM_008367
• Protein Refseq: NP_032393
• UniProt ID: P01590