| Species : |
Mouse |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
1-235 aa |
| Form : |
Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
1. Immobilized Recombinant Rat CSF1R / MCSF Receptor / CD115 Protein (Fc Tag) at 2 μg/ml (100 μl/well) can bind Recombinant Mouse IL-34 Protein (His Tag), the EC50 is 1.5-4.5 ng/mL. 2. Measured in a cell proliferation assay using M-NFS-60 mouse myeloid cells. The ED50 for this effect is typically 50-200 ng/mL. |
| Molecular Mass : |
26 kDa |
| Endotoxin : |
< 1 EU/μg of the protein by LAL method. |
| Purity : |
≥ 90 % as determined by SDS-PAGE. |
| Storage : |
Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. |
| Reconstitution : |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
