Recombinant Mouse Mgmt Protein, Myc/DDK-tagged

• Cat.No.: Mgmt-4062M
• Product Overview: Purified recombinant protein of mouse full-length O-6-methylguanine-DNA methyltransferase (Mgmt), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
• Molecular Mass: 22.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mgmt O-6-methylguanine-DNA methyltransferase [ Mus musculus (house mouse) ]
• Official Symbol: Mgmt
• Synonyms: MGMT; O-6-methylguanine-DNA methyltransferase; methylated-DNA--protein-cysteine methyltransferase; O-6-alkylguanine-DNA alkyltransferase; O-6-methylguanine-DNA-alkyltransferase; 6-O-methylguanine-DNA methyltransferase; AGT; Agat; AI267024; MGC107020
• Gene ID: 17314
• mRNA Refseq: NM_008598
• Protein Refseq: NP_032624
• UniProt ID: P26187