Recombinant Mouse Stat3 Protein, Myc/DDK-tagged

• Cat.No.: Stat3-6170M
• Product Overview: Purified recombinant protein of mouse full-length signal transducer and activator of transcription 3 (Stat3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 88.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Stat3 signal transducer and activator of transcription 3 [ Mus musculus (house mouse) ]
• Official Symbol: Stat3
• Synonyms: STAT3; signal transducer and activator of transcription 3; acute phase response factor; acute-phase response factor; Aprf; AW109958; 1110034C02Rik
• Gene ID: 20848
• mRNA Refseq: NM_213659
• Protein Refseq: NP_998824
• UniProt ID: P42227