Recombinant Mouse TF protein, Mouse IgG2a Fc-tagged, low endotoxin TF-379M

Recombinant Mouse TF protein, Mouse IgG2a Fc-tagged, low endotoxin

PRODUCTS

Home / Products / Recombinant Proteins / Recombinant Mouse TF protein, Mouse IgG2a Fc-tagged, low endotoxin

Recombinant Mouse TF protein, Mouse IgG2a Fc-tagged, low endotoxin

TF Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat.No. : TF-379M
Product Overview : Recombinant Mouse TF protein (Val20-His697)(low endotoxin), fused to Mouse IgG2a Fc tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : Transferrin is also known as Serotransferrin, Beta-1 metal-binding globulin, TF, and is iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h). The affinity of transferrin for Fe(III) is extremely high (1023 M−1 at pH 7.4) but decreases progressively with decreasing pH below neutrality.When not bound to iron, it is known as "apo-transferrin”. In humans, transferrin consists of a polypeptide chain containing 679 amino acids. It is a complex composed of alpha helices and beta sheets to form two domains (the first situated in the N-terminus and the second in the C-terminus). The N- and C- terminal sequences are represented by globular lobes and between the two lobes is an iron-binding site. The liver is the main source of manufacturing transferrin, but other sources such as the brain also produce this molecule. Transferrin is also associated with the innate immune system. Transferrin is found in the mucosa and binds iron, thus creating an environment low in free iron that impedes bacteria survival in a process called iron withholding. The level of transferrin decreases in inflammation. The metal binding properties of transferrin have a great influence on the biochemistry of plutonium in humans. Transferrin has a bacteriocidal effect on bacteria, in that it makes Fe3+ unavailable to the bacteria. Carbohydrate deficient transferrin increases in the blood with heavy ethanol consumption and can be monitored via laboratory testing.
Source : HEK293
Species : Mouse
Tag : Mouse IgG2a Fc
Form : Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 101.8 kDa. The protein migrates as 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : 678
Endotoxin : Less than 0.1 EU per ug by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of TF-379M :

Mouse Transferrin, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of TF-379M :

Immobilized Human TfR, His Tag at 2 μg/mL (100 μL/well) can bind Mouse Transferrin, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.4-12.5 ng/mL (QC tested).

Gene Name : Trf
Official Symbol : Trf
Synonyms : HP; Tf; Tfn; hpx; Cd176; AI266983
Gene ID : 22041
mRNA Refseq : NM_133977.2
Protein Refseq : NP_598738.1
UniProt ID : Q921I1
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry


  • Note: There will be extra charge for optional service!

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2022 Creative BioMart. All Rights Reserved. Terms and Conditions | Privacy Policy