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Recombinant Mouse TGFBR3 cell lysate

Cat.No. : TGFBR3-1847MCL
Product Overview : Mouse TGFBR3 / Betaglycan derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : Tgfbr3 transforming growth factor, beta receptor III [ Mus musculus ]
Official Symbol : TGFBR3
Synonyms : TGFBR3; transforming growth factor, beta receptor III; transforming growth factor beta receptor type 3; TGFR-3; betaglycan; TGF-beta receptor type 3; TGF-beta receptor type III; TBRIII; AU015626; AW215636; 1110036H20Rik;
Gene ID : 21814
mRNA Refseq : NM_011578
Protein Refseq : NP_035708
Pathway : TGF Beta Signaling Pathway, organism-specific biosystem; TGF-beta Receptor Signaling Pathway, organism-specific biosystem;
Function : PDZ domain binding; SMAD binding; SMAD binding; activin binding; coreceptor activity; fibroblast growth factor 2 binding; glycosaminoglycan binding; heparin binding; protein binding; receptor activity; transforming growth factor beta binding; transforming growth factor beta binding; transforming growth factor beta receptor activity, type III; transforming growth factor beta receptor binding; transforming growth factor beta-activated receptor activity; transforming growth factor beta-activated receptor activity; transforming growth factor beta-activated receptor activity; type II transforming growth factor beta receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What are the implications of TGFBR3 mutations in the context of hereditary connective tissue disorders? 03/05/2022

TGFBR3 mutations are associated with hereditary connective tissue disorders, impacting extracellular matrix homeostasis and contributing to conditions like Loeys-Dietz syndrome.

How does TGFBR3 influence embryonic development, and what are the consequences of its dysregulation during development? 04/26/2021

TGFBR3 plays a role in embryonic development, and dysregulation may lead to developmental abnormalities due to disrupted TGF-beta signaling.

Can you explain the role of TGFBR3 in mediating interactions between cells and extracellular matrix components? 07/15/2019

TGFBR3 facilitates cell-ECM interactions by modulating TGF-beta signaling, influencing processes like cell adhesion and migration.

How does TGFBR3 contribute to the modulation of TGF-beta signaling pathways? 05/24/2018

TGFBR3 acts as a co-receptor, enhancing the binding of TGF-beta ligands to TGF-beta receptors, and modulates downstream signaling events.

What are the functional consequences of TGFBR3 mutations in the context of cardiovascular diseases? 02/11/2018

TGFBR3 mutations are implicated in cardiovascular diseases, affecting TGF-beta-mediated processes in vascular smooth muscle cells and cardiac tissues.

Customer Reviews (3)

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05/10/2022

    Great product !

    02/18/2020

      Seamless delivery process—ordered, received, and ready for experiments.

      08/31/2018

        Timely delivery is key, and this product arrived on schedule.

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