Recombinant Mouse Uba1 Protein, Myc/DDK-tagged

• Cat.No.: Uba1-6764M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin-like modifier activating enzyme 1 (Uba1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes a member of the ubiquitin-activating E1 family. The encoded protein initiates the ubiquitin activation and transfer cascade, catalyzing the first step in ubiquitin conjugation to mark cellular proteins for proteasome degradation. Ubiquitin activating enzymes use ATP to form a thioester between a conserved catalytic cysteine of the enzyme and the C-terminal carboxylate of ubiquitin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 117.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Uba1 ubiquitin-like modifier activating enzyme 1 [ Mus musculus (house mouse) ]
• Official Symbol: Uba1
• Synonyms: UBA1; ubiquitin-like modifier activating enzyme 1; ubiquitin-like modifier-activating enzyme 1; ubiquitin-activating enzyme E1 X; ubiquitin-activating enzyme E1, Chr X; ubiquitin-like modifier-activating enzyme 1 X; Sbx; A1S9; Ube-1; Ube1x; AA989744
• Gene ID: 22201
• mRNA Refseq: NM_001136085
• Protein Refseq: NP_001129557
• UniProt ID: Q02053

Reviews

07/02/2022

The UBA1 protein comes highly recommended for its outstanding performance in various experimental applications.

07/07/2019

In ELISA assays, it has consistently exhibited exceptional reliability and accuracy, providing precise and reproducible results.

05/13/2017

Their prompt and knowledgeable guidance has been instrumental in effectively addressing my queries and optimizing the utilization of the UBA1 protein in my studies.

Q&As

Are there any clinical trials involving UBA1 as a target?

Some early-phase clinical trials are exploring UBA1 as a potential target in the context of ALS and cancer.

How can UBA1 be targeted for therapeutic purposes?

Targeting UBA1 with small molecules or gene therapy may provide a potential avenue for treating UBA1-related diseases.

How does UBA1 function in the cell?

UBA1 activates ubiquitin, a small protein that tags other proteins for degradation by the proteasome.

What diseases are associated with UBA1 dysfunction?

UBA1 dysfunction has been linked to neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) and certain types of cancer.

Can UBA1 be a diagnostic marker for ALS?

Research suggests that altered UBA1 levels in the cerebrospinal fluid may be a potential biomarker for ALS diagnosis.