Recombinant Rat CD6 protein(Met1-Asn396), His-tagged

• Cat.No.: CD6-1144R
• Product Overview: Recombinant Rat CD6 (Q812A4) (Met1-Asn396) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Protein length: Met1-Asn396
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Immobilized rat CD6-His at 10 μg/mL (100 μL/well) can bind rat ALCAM-Fc3, The EC50 of rat ALCAM-Fc3 is 19.5-45.5 ng/ml. 2. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×10E4 cells/well are added to ratCD6-His coated plates (5 μg/mL and 100 μL/well) in the ppresence of 10μg/ml PHA, approximately > 50% cells will adhere specifically after 60 minutes at 37°C.
• Molecular Mass: The recombinant rat CD6 comprises 390 amino acids and predicts a molecular mass of 42.5 kDa. The apparent molecular mass of the recombinant protein is approximately 64-70 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd6 Cd6 molecule [ Rattus norvegicus ]
• Official Symbol: CD6
• Synonyms: CD6; Cd6 molecule; CD6 antigen; CD6 antigen, Tp120; OX52; MGC108551
• Gene ID: 25752
• mRNA Refseq: NM_175577
• Protein Refseq: NP_783167

Reviews

04/05/2021

Fantastic customer support. They genuinely care about the success of our research.

11/11/2020

Quality you can trust. Our lab has seen improved precision since using this product.

07/27/2019

Shipping is always on time, and the packaging ensures the product arrives in perfect condition.

Q&As

Can you explain the role of CD6 in T-cell co-stimulation and its implications for adaptive immune responses?

CD6 provides co-stimulatory signals to T cells, influencing their activation and differentiation. This contributes to the regulation of adaptive immune responses.

How are CD6 genes polymorphic, and what is the significance of genetic variations in the context of autoimmune diseases?

CD6 genes exhibit polymorphisms, and certain variations are associated with susceptibility to autoimmune diseases, indicating a potential role in immune dysregulation.

How does CD6 contribute to the formation of immunological synapses, and what molecular events are involved in this process?

CD6 is involved in immunological synapse formation, and its interactions with ligands, such as ALCAM, play a role in stabilizing T-cell interactions with antigen-presenting cells.

How does CD6 influence T-cell trafficking and migration, and what are the implications for immune surveillance and inflammation?

CD6 modulates T-cell trafficking and migration, impacting immune surveillance and inflammation. Its interactions with adhesion molecules contribute to these processes.

What is the relationship between CD6 and antigen-presenting cells, and how does it impact the initiation of immune responses?

CD6 interacts with antigen-presenting cells, facilitating T-cell activation. This interaction is pivotal for the initiation and regulation of immune responses.