Recombinant Rat Il12a protein, His & T7-tagged
Cat.No. : | Il12a-853R |
Product Overview : | Recombinant Rat Il12a aa. (Arg23~Ser215 (Accession# Q9R103)) fused with N-terminal His & T7 tag was produced in E. coli cells. |
- Specification
- Gene Information
- Related Products
Description : | Mouse homolog is a subunit of heteromeric cytokine interleukin 12; loss of function is associated with experimental autoimmune encephalomyelitis. |
Source : | E. coli |
Species : | Rat |
Tag : | His & T7 |
Form : | Freeze-dried powder |
Molecular Mass : | Predicted Molecular Mass: 25.5kDa |
Protein length : | Arg23~Ser215 (Accession# Q9R103) |
Endotoxin : | <1.0EU per 1µg (determined by the LAL method) |
Purity : | > 95% |
Characteristic : | The isoelectric point is 7.8. |
Applications : | SDS-PAGE; WB; ELISA; IP |
Stability : | The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Storage buffer : | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl |
Reconstitution : | Reconstitute in sterile PBS, pH7.2-pH7.4 |
Gene Name : | Il12a interleukin 12A [ Rattus norvegicus (Norway rat) ] |
Official Symbol : | Il12a |
Synonyms : | Il12a; interleukin 12A; interleukin-12 subunit alpha; CLMF p35; IL-12 subunit p35; IL-12A; cytotoxic lymphocyte maturation factor 35 kDa subunit; interleukin 12 p35 subunit |
Gene ID : | 84405 |
mRNA Refseq : | NM_053390.1 |
Protein Refseq : | NP_445842.1 |
UniProt ID : | Q9R103 |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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- Reviews
Q&As (10)
Ask a questionMass spectrometry identifies IL12A's post-translational modifications and their roles.
Knockout models validate IL12A's roles in immune response modulation.
Protein footprinting assays reveal IL12A's binding interfaces with receptors.
ELISA and stability assays quantify IL12A secretion and stability in culture.
SPR characterizes IL12A's interactions with antibodies and inhibitors.
Cellular assays gauge IL12A's cytokine induction in immune cells.
Isothermal titration calorimetry measures IL12A's binding affinity with receptors.
Structural biology techniques analyze IL12A genetic variant impacts.
Site-directed mutagenesis probes IL12A's functional domains and interactions.
Bioluminescence imaging tracks IL12A expression dynamics in vivo.
Customer Reviews (3)
Write a reviewSuitable for co-immunoprecipitation.
Consistent in bioactivity.
Good for receptor binding studies.
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