Active Recombinant Rat Il25 protein(Val17-Ala169), hFc-tagged

• Cat.No.: Il25-8736R
• Product Overview: Recombinant Rat IL25 (D3ZLB1) (Val17-Ala169) was expressed in HEK293, fused with Fc region of Human IgG1 at the N-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: N-hFc
• Protein length: Val17-Ala169
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse IL17BR-His at 10 μg/mL (100 μl/well) can bind Rat Fc-IL25. The EC50 of Rat Fc-IL25 is 0.12-0.27 μg/mL.
• Molecular Mass: The recombinant Rat IL25 comprises 413 amino acids and predicts a molecular mass of 46.1 kDa. The apparent molecular mass of the recombinant protein is approximately 49 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il25 interleukin 25 [ Rattus norvegicus ]
• Official Symbol: Il25
• Synonyms: IL25; interleukin 25; interleukin-25; RGD1561632
• Gene ID: 501996
• mRNA Refseq: NM_001192007
• Protein Refseq: NP_001178936