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Recombinant Rhesus macaque CD4 protein, His-tagged

Cat.No. : CD4-377R
Product Overview : Recombinant Rhesus macaque CD4 protein(G7N5T8)(Lys26-Trp390), fused with C-terminal His tag, was expressed in HEK293.
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Source : HEK293
Species : Rhesus macaque
Tag : C-His
Protein length : Lys26-Trp390
Form : Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Rhesus macaque CD4, His Tag at 1 μg/ml (100 μl/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 of 13.0 ng/ml determined by ELISA.
Molecular Mass : The protein has a predicted MW of 41.5 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Tris-Bis PAGE result.
Endotoxin : Less than 1EU per μg by the LAL method.
Purity : > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Storage : Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Reconstitution : Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Not For Human Consumption!

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Customer Reviews (3)

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Reviews
01/08/2031

    The CD4 proteins purchased from your company is suitable for ELISA.

    02/06/2023

      Protein products are put into experiments with good effect and reliable experimental results.

      01/22/2022

        The technical expert will follow up on the progress of my experiments and provide constructive advice on the problems that arise in my experiments.

        Q&As (3)

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        What is the connection between CD4 and glycoprotein GP120? 11/09/2022

        CD4 binds to glycoprotein GP120.

        Where is CD4 primarily expressed? 02/26/2022

        CD4 is mainly expressed in T cells and thymocytes.

        Where is CD4 localized? 03/14/2021

        CD4 is localized in the cell membrane.

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