Active Recombinant Feline CD4, His-tagged

• Cat.No.: CD4-634F
• Product Overview: Recombinant Feline CD4 (Accession # NP_001009250) Met1-Leu413 (Lys20Glu, Ser84Cys, Val103Ile, Asn115Asp, Ser142Thr, Ser169Gly & insertions of SerThr between Thr148 and Ser149 ), fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Feline
• Tag: His
• Predicted N Terminal: Ala22
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.The ED50 for this effect is typically 0.1 - 0.5 µg/mL after a 1 hour incubation at 37 °C. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Feline CD4, His-tagged has a calculated MW of 43.3 kDa. In SDS-PAGE migrates as 45-55 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: CD4 CD4 molecule [ Felis catus (domestic cat) ]
• Official Symbol: CD4
• Synonyms: CD4; T-cell surface glycoprotein CD4; CD4 antigen; CD4 molecule
• Gene ID: 493775
• mRNA Refseq: NM_001009250
• Protein Refseq: NP_001009250
• Chromosome Location: B4