| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
868-1231 aa |
| Description : |
Complement Factor H (CFH) composed of 20 repetitive units of 60 amino acids, named short consensus repeats (SCR) or complement control protein modules (CCP), arranged in a continuous fashion. CFH is amongst the most abundant complement components in human blood, where the liver is the major source of CFH protein in vivo. The CFH molecule includes different interaction sites for C3b and polyanions which delineate distinct functional domains at the N- and C-termini. The C3b binding site in SCR1-4 is the only site essential for the factor I co-factor activity of factor H. Similarly, the C3b/polyanions-binding site located within SCR19-20 is the most important site for preventing alternative pathway activation on host cells. Mutations in the CFH gene are associated with severe and diverse diseases including the rare renal disorders hemolytic uremic syndrome (HUS) and membranoproliferative (MPGN) also termed dense deposit disease (DDD), as well as the more frequent retinal disease age related macular degeneration (AMD). |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
| Bio-activity : |
Immobilized Human CFH Protein, His Tag at 10 μg/mL (100 μL/well) can bind Heparin Biotin with a linear range of 0.01-1.25 μg/mL. |
| Molecular Mass : |
The protein has a calculated MW of 42.7 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Purity : |
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS. |
| Storage : |
This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
