Active Recombinant Human FGF19 Protein (Arg23-Lys216), C-His tagged, Animal-free, Carrier-free

• Cat.No.: FGF19-325H
• Product Overview: Recombinant Human FGF19 Protein (Arg23-Lys216) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Fibroblast Growth Factors-19 (FGF-19) is a 24 kDa member of the fibroblast Growth Factors with 216 amino acid residues. FGF-19 is mainly secreted from cholangiocytes, ductal cells. FGF-19 involved cell proliferation, glucose and bile acid metabolism via FGFR4 pathway.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 51 ng/mL.
• Molecular Mass: The protein has a calculated MW of 22.62 kDa.
  The protein migrates as 24 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Arg23-Lys216
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: FGF19 fibroblast growth factor 19 [ Homo sapiens (human) ]
• Official Symbol: FGF19
• Synonyms: FGF19; fibroblast growth factor 19; FGF-19
• Gene ID: 9965
• mRNA Refseq: NM_005117
• Protein Refseq: NP_005108
• MIM: 603891
• UniProt ID: O95750

Reviews

01/14/2022

Top-notch protein quality! It's our go-to for reliable and reproducible results.

09/08/2020

This product simplifies our research. It's a reliable tool that we can count on.

05/11/2018

If you're in the lab, you need this product. It streamlines processes and is a lab necessity.

Q&As

How is FGF19 gene expression regulated, and what factors influence its secretion and activity in various tissues?

FGF19 expression is regulated by bile acids and other factors. Its secretion and activity are influenced by nutrient availability, signaling pathways, and interactions with bile acid receptors.

Can you discuss the potential therapeutic applications of targeting FGF19 in metabolic disorders, and what challenges exist in developing such interventions?

Targeting FGF19 holds therapeutic potential for metabolic disorders. Challenges include achieving tissue-specific effects and understanding the long-term consequences of manipulating FGF19 signaling.

Can you explain the role of FGF19 in the enterohepatic circulation of bile acids and its implications for metabolic health?

FGF19 regulates bile acid synthesis in the liver and influences the enterohepatic circulation, impacting metabolic health by modulating lipid and glucose metabolism.

What is the role of FGF19 in the regulation of gut hormone secretion, and how does this contribute to its effects on metabolic processes?

FGF19 influences gut hormone secretion, including the release of glucagon-like peptide-1 (GLP-1). This regulation contributes to its metabolic effects, such as enhanced insulin sensitivity and reduced food intake.

What is the significance of FGF19 in metabolic diseases, and how does dysregulation contribute to conditions like obesity and non-alcoholic fatty liver disease (NAFLD)?

Dysregulation of FGF19 is implicated in metabolic diseases, including obesity and NAFLD. Altered FGF19 signaling may contribute to aberrant bile acid and lipid metabolism in these conditions.