Active Recombinant Human IL1RN Protein
|Product Overview :||Recombinant mature form of human IL1RA isoform1 (NP_776214.1) (Arg 26-Glu 177) was expressed in E. coli.|
- Gene Information
- Related Products
|Source :||E. coli|
|Form :||Lyophilized from sterile 50 mM Tris, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
|Bio-activity :||1. Measured by its binding ability in a functional ELISA. Immobilized human IL1RA at 10 μg/ml (100 μl/well) can bind human IL1R2-Fc, The EC50 of human IL1R2-Fc is 0.04-0.1 μg/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized human IL1RA at 10 μg/ml (100 μl/well) can bind human IL1R1-Fc, The EC50 of human IL1R1-Fc is 0.08-0.2 μg/mL.
3. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells in the presence of 250pg/mL IL1a. The EC50 for this effect is typically 3-12 ng/mL.
|Molecular Mass :||The recombinant human IL1RA consists of 153 amino acids and predicts a molecular mass of 17.3 kDa which is also estimated by SDS-PAGE.|
|Protein Length :||Arg 26-Glu 177|
|Endotoxin :||< 1.0 EU per μg protein as determined by the LAL method.|
|Purity :||> 97 % as determined by SDS-PAGE|
|Storage :||Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 68°C before opening to recover the entire contents.|
|Gene Name :||IL1RN interleukin 1 receptor antagonist [ Homo sapiens ]|
|Official Symbol :||IL1RN|
|Synonyms :||IL1RN; interleukin 1 receptor antagonist; interleukin-1 receptor antagonist protein; ICIL 1RA; IL 1RN; IL1F3; IL1RA; interleukin 1 receptor antagonist protein; intracellular interleukin 1 receptor antagonist; IRAP; MGC10430; IL1 inhibitor; IL1RN (IL1F3); type II interleukin-1 receptor antagonist; intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra); DIRA; MVCD4; IL-1RN; IL-1ra; IL-1ra3; ICIL-1RA;|
|Gene ID :||3557|
|mRNA Refseq :||NM_000577|
|Protein Refseq :||NP_000568|
|UniProt ID :||P18510|
|◆ Recombinant Protein|
|Il1rn-1244M||Recombinant Mouse Il1rn Protein, MYC/DDK-tagged||+Inquiry|
|IL1RN-362C||Recombinant Cynomolgus Monkey IL1RN Protein, His (Fc)-Avi-tagged||+Inquiry|
|IL1RN-1171H||Recombinant Human IL1RN Protein, His (Fc)-Avi-tagged||+Inquiry|
|IL1RN-104I||Active Recombinant Human IL1RN Protein (153 aa)||+Inquiry|
|IL1RN-1819H||Recombinant Human IL1RN Protein, MYC/DDK-tagged||+Inquiry|
|IL1RN-2912HCL||Recombinant Human IL1RN cell lysate||+Inquiry|
|IL1RN-1375RCL||Recombinant Rat IL1RN cell lysate||+Inquiry|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.