Active Recombinant Human Interleukin 17 Receptor C, HIgG1 Fc-tagged

• Cat.No.: IL17RC-645H
• Product Overview: The extracellular domain of human IL-17RC (NP_116121.2)(Leu21-Ala454) is fused to the N-terminus of the Fc region of a human IgG1 was expressed in CHO cell.

Specification

• Description: This gene encodes a single-pass type I membrane protein that shares similarity with the interleukin-17 receptor (IL-17RA). Unlike IL-17RA, which is predominantly expressed in hemopoietic cells, and binds with high affinity to only IL-17A, this protein is expressed in nonhemopoietic tissues, and binds both IL-17A and IL-17F with similar affinities. The proinflammatory cytokines, IL-17A and IL-17F, have been implicated in the progression of inflammatory and autoimmune diseases. Multiple alternatively spliced transcript variants encoding different isoforms have been detected for this gene, and it has been proposed that soluble, secreted proteins lacking transmembrane and intracellular domains may function as extracellular antagonists to cytokine signaling.
• Source: CHO cell
• Species: Human
• Tag: HIgG1 Fc-tagged
• Form: Lyophilized from 0.2μm-filtered solution in PBS.
• Bio-activity: Measured by its ability to bind rhIL-17 in a functional ELISA.
• Molecular Mass: 74KDa (monomer)
• AA Sequence: Leu21-Ala454
• Endotoxin: <0.06 eu/μg as determined by lal>
• Purity: >98%, by SDS-PAGE under reducing conditions.
• Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
• Reconstitution: Reconstitute at 100μg/ml in sterile PBS.
• Warning: Avoid freeze/thaw cycles.
• Protein length: Leu21-Ala454

Gene Information

• Gene Name: IL17RC interleukin 17 receptor C [ Homo sapiens ]
• Official Symbol: IL17RC
• Synonyms: IL17RC; interleukin 17 receptor C; interleukin-17 receptor C; IL17 RL; IL-17RL; IL17Rhom; zcytoR14; IL17F receptor; IL-17 receptor C; interleukin-17 receptor homolog; interleukin-17 receptor-like protein; IL17RL; IL17-RL; FLJ95963; FLJ96005; MGC10763
• Gene ID: 84818
• mRNA Refseq: NM_001203263
• Protein Refseq: NP_001190192
• MIM: 610925
• UniProt ID: Q8NAC3
• Chromosome Location: 3p25.3
• Function: receptor activity

Reviews

12/11/2022

High purity.

06/19/2022

Suitable for signaling studies.

06/11/2021

Effective in neutrophil activation.

Q&As

What experimental strategies are employed to investigate the crosstalk between IL17RC and other immune receptors or cytokine pathways?

Co-stimulation assays, co-immunoprecipitation, and gene expression analysis reveal IL17RC's interplay with other immune pathways.

How are structural biology approaches, such as X-ray crystallography or cryo-electron microscopy, used to understand IL17RC's interaction with its ligands?

Structural studies provide insights into IL17RC's binding interface with ligands, aiding in understanding cytokine-receptor interactions.

What methodologies are used to assess the impact of IL17RC inhibition on immune cell differentiation, migration, and cytokine production?

In vitro cell migration assays, cytokine profiling, and adoptive transfer experiments investigate IL17RC's impact on immune cell functions.

Can you elaborate on the methods used to investigate IL17RC's contribution to autoimmune diseases, considering its involvement in IL17-mediated inflammation?

Animal models, patient-derived cells, and genetic association studies explore IL17RC's involvement in autoimmune disorders.

Could you explain how genetic and pharmacological manipulation of IL17RC expression or function in animal models helps elucidate its role in tissue-specific inflammation?

Conditional knockout models, adoptive transfer experiments, and tissue-specific inflammation assays unveil IL17RC's role in disease contexts.

How are patient-derived samples and clinical data integrated into experimental research to investigate the relevance of IL17RC in human diseases?

Biomarker analysis, patient stratification, and correlation studies validate the clinical relevance of IL17RC in human diseases.

How do researchers study the potential of targeting IL17RC as a therapeutic strategy in inflammatory and autoimmune conditions?

Preclinical models, therapeutic antibodies, and disease amelioration studies assess IL17RC as a target for immune-mediated disorders.

What cellular and molecular assays are utilized to unravel the downstream signaling cascades triggered by IL17RC activation and their impact on immune responses?

Phosphorylation assays, reporter gene assays, and CRISPR screens dissect IL17RC-mediated signaling events and immune outcomes.

What experimental techniques are employed to study the expression and localization of IL17RC in different immune and non-immune cell types?

Immunofluorescence, flow cytometry, and single-cell RNA sequencing determine IL17RC expression profiles in diverse cell populations.

How do researchers experimentally distinguish the roles of IL17RC in specific IL17 cytokine signaling pathways?

Perturbation studies, siRNA knockdown, and gene editing differentiate IL17RC's roles in distinct IL17 cytokine pathways.