Recombinant Human IL17RC cell lysate

Cat.No. : IL17RC-1611HCL
Product Overview : Human IL17RC derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : IL17RC interleukin 17 receptor C [ Homo sapiens ]
Official Symbol : IL17RC
Synonyms : IL17RC; interleukin 17 receptor C; interleukin-17 receptor C; IL17 RL; IL-17RL; IL17Rhom; zcytoR14; IL17F receptor; IL-17 receptor C; interleukin-17 receptor homolog; interleukin-17 receptor-like protein; IL17RL; IL17-RL; FLJ95963; FLJ96005; MGC10763;
Gene ID : 84818
mRNA Refseq : NM_001203263
Protein Refseq : NP_001190192
MIM : 610925
UniProt ID : Q8NAC3
Chromosome Location : 3p25.3
Function : receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What experimental strategies are employed to investigate the crosstalk between IL17RC and other immune receptors or cytokine pathways? 06/15/2022

Co-stimulation assays, co-immunoprecipitation, and gene expression analysis reveal IL17RC's interplay with other immune pathways.

How are structural biology approaches, such as X-ray crystallography or cryo-electron microscopy, used to understand IL17RC's interaction with its ligands? 02/02/2022

Structural studies provide insights into IL17RC's binding interface with ligands, aiding in understanding cytokine-receptor interactions.

What methodologies are used to assess the impact of IL17RC inhibition on immune cell differentiation, migration, and cytokine production? 08/11/2021

In vitro cell migration assays, cytokine profiling, and adoptive transfer experiments investigate IL17RC's impact on immune cell functions.

Can you elaborate on the methods used to investigate IL17RC's contribution to autoimmune diseases, considering its involvement in IL17-mediated inflammation? 04/15/2021

Animal models, patient-derived cells, and genetic association studies explore IL17RC's involvement in autoimmune disorders.

Could you explain how genetic and pharmacological manipulation of IL17RC expression or function in animal models helps elucidate its role in tissue-specific inflammation? 05/26/2020

Conditional knockout models, adoptive transfer experiments, and tissue-specific inflammation assays unveil IL17RC's role in disease contexts.

How are patient-derived samples and clinical data integrated into experimental research to investigate the relevance of IL17RC in human diseases? 11/23/2019

Biomarker analysis, patient stratification, and correlation studies validate the clinical relevance of IL17RC in human diseases.

How do researchers study the potential of targeting IL17RC as a therapeutic strategy in inflammatory and autoimmune conditions? 05/04/2018

Preclinical models, therapeutic antibodies, and disease amelioration studies assess IL17RC as a target for immune-mediated disorders.

What cellular and molecular assays are utilized to unravel the downstream signaling cascades triggered by IL17RC activation and their impact on immune responses? 11/23/2017

Phosphorylation assays, reporter gene assays, and CRISPR screens dissect IL17RC-mediated signaling events and immune outcomes.

What experimental techniques are employed to study the expression and localization of IL17RC in different immune and non-immune cell types? 09/29/2017

Immunofluorescence, flow cytometry, and single-cell RNA sequencing determine IL17RC expression profiles in diverse cell populations.

How do researchers experimentally distinguish the roles of IL17RC in specific IL17 cytokine signaling pathways? 07/06/2017

Perturbation studies, siRNA knockdown, and gene editing differentiate IL17RC's roles in distinct IL17 cytokine pathways.

Customer Reviews (3)

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Reviews
12/11/2022

    High purity.

    06/19/2022

      Suitable for signaling studies.

      06/11/2021

        Effective in neutrophil activation.

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