Active Recombinant Human S100A9 protein, His-tagged

• Cat.No.: S100A9-300H
• Product Overview: Active Recombinant Human S100A9 protein(NP_002956.1) (Met 1-Pro 114) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-114 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: 1. Measured by its ability to bind recombinant human S100A8-his in a functional ELISA. 2. Measured by its ability to bind recombinant human his-S100A8 in a functional ELISA. 3. Measured by its ability to inhibit the proliferation of MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 10-30 μg/mL.
• Molecular Mass: The recombinant human S100A9 consists of 124 amino acids and predicts a molecular mass of 14.6 kDa. It migrates as an approximately 16 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_002956.1
• Unit ID: P06702
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/P06702

Gene Information

• Gene Name: S100A9 S100 calcium binding protein A9 [ Homo sapiens ]
• Official Symbol: S100A9
• Synonyms: S100A9; S100 calcium binding protein A9; CAGB, CFAG, S100 calcium binding protein A9 (calgranulin B), S100 calcium binding protein A9 (calgranulin B); protein S100-A9; 60B8AG; CGLB; LIAG; MAC387; MIF; MRP14; NIF; P14; MRP-14; calgranulin B; calgranulin-B; calprotectin L1H subunit; leukocyte L1 complex heavy chain; migration inhibitory factor-related protein 14; S100 calcium-binding protein A9 (calgranulin B); CAGB; CFAG; L1AG
• Gene ID: 6280
• mRNA Refseq: NM_002965
• protein Refseq: NP_002956
• MIM: 123886