Active Recombinant Mouse Il27 Protein, His-Tagged

• Cat.No.: Il27-01M
• Product Overview: Recombinant mouse Il27 Protein, His-tagged was expressed in E. coli cell

Specification

• Description: IL-27 protein is a member of the IL-6 superfamily, which is expressed on monocytes, endothelial cells and dendritic cells. It is also referred to as the IL-12 p35-related protein. IL-27 protein is an early product of activated antigen-presenting cells and drives rapid clonal expansion of naive CD4(+) T cells and plays a role in the early regulation of Th1 cells initiation which drives efficient adaptive immune response. It has an antiproliferative activity on melanomas through WSX-1/STAT1 signaling. Thus, IL-27 protein may be an attractive candidate as an antitumor agent applicable to cancer immunotherapy.
• Source: Escherichia coli
• Species: Mouse
• Tag: His
• Form: Lyophilized powder
• AA Sequence: MALVALSQPRVQCHASRYPVAVDCS WTPLQAPNSTRSTSFIATYRLGVAT QQQSQPCLQRSPQASRCTIPDVHLF STVPYMLNVTAVHPG
  GASSSLLAFVAERIIKPDPPEGVRL RTAGQRLQVLWHPPASWPFPDIFSL KYRLRYRRRGASHFRQVGPIEATTF TLRNSKPHAKYCIQVSAQ
  DLTDYGKPSDWSLPGQVESAPHKP with polyhistidine tag at the C-terminus
• Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
• Bio-activity: Measure by its ability to protect HepG2 cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is <5 ng/mL
• Purity: >95% as determined by SDS-PAGE. Ni-NTA chromatography
• Storage Buffer: The protein was lyophilized from a solution containing 20 mM sodium carbonate, 0.2M NaCl, pH 4.5.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Storage: Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
• Notes: Please use within one month after protein reconstitution.

Gene Information

• Gene Name: Il27 interleukin 27 [ Mus musculus (house mouse) ]
• Official Symbol: Il27
• Synonyms: p28; Il30; IL-27; IL27-A; IL-27-A; IL-27p28
• Gene ID: 246779
• mRNA Refseq: NM_145636.2
• Protein Refseq: NP_663611.1
• UniProt ID: Q8K3I6

Reviews

03/30/2022

The purity is very high, no impurities.

08/05/2020

Biological activity is very strong and can be used in various biological experiments.

05/30/2019

Stability is very good, and it will not denaturate at high temperatures.

Q&As

Are there have current difficulties in the study of Il27?

The current challenges in the study of Il27 include potential side effects, preparation purity and other challenges.

Which scientific research fields are deeply studying Il27?

The potential application of Il27 in tumor immunotherapy and autoimmune diseases is being deeply studied in the scientific research field.

Is the production of Il27 related to genetic factors?

The production of Il27 is related to genetic factors, and the expression level and polymorphism of genes may affect its production and function.

Which role of Il27 in inflammatory diseases?

Studies have shown that Il27 may have an anti-inflammatory effect in inflammatory diseases, regulating the degree of inflammatory response.

What the potential applications of recombinant Il27 in cancer therapy?

Current studies have shown that Il27 has potential application value in enhancing anti-tumor immunity and alleviating tumor invasion and metastasis.

What is the effect of Il27 on immune response?

Il27 can enhance the activation and proliferation of specific immune cells and regulate the intensity and direction of immune response.