Recombinant Active Mouse IL27 Protein, His-tagged(C-ter)

• Cat.No.: Il27-185M
• Product Overview: Recombinant Active Mouse IL27 Protein with His tag (C-ter) was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR). [provided by RefSeq, Jul 2008]
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Powder
• Bio-activity: Determined by its ability to protect HepG2 cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is < 5 ng/mL.
• AA Sequence: MFPTDPLSLQELRREFTVSLYLARK LLSEVQGYVHSFAESRLPGVNLDLL PLGYHLPNVSLTFQAWHHLSDSERL CFLATTLRPFPAMLGGLGTQGTWTS SEREQLWAMRLDLRDLHRHLRFQVL AAGFKCSKEEEDKEEEEEEEEEEKK LPLGALGGPNQVSSQVSWPQLLYTY QLLHSLELVLSRAVRDLLLLSLPRR PGSAWDS
• Endotoxin: Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test.
• Purity: > 98% (by SDS-PAGE)
• Applications: SDS-PAGE
• Notes: For laboratory research only, not for drug, diagnostic or other use.
• Storage: Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
• Storage Buffer: PBS (pH 7.4)
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.

Gene Information

• Gene Name: Il27 interleukin 27 [ Mus musculus ]
• Official Symbol: Il27
• Synonyms: IL27; interleukin 27; interleukin-27 subunit alpha; IL27-A; IL-27-A; interleukin 30; IL-27 p28 subunit; IL-27 subunit alpha; p28; Il30; IL-27; IL-27p28
• Gene ID: 246779
• mRNA Refseq: NM_145636
• Protein Refseq: NP_663611

Reviews

03/30/2022

The purity is very high, no impurities.

08/05/2020

Biological activity is very strong and can be used in various biological experiments.

05/30/2019

Stability is very good, and it will not denaturate at high temperatures.

Q&As

Are there have current difficulties in the study of Il27?

The current challenges in the study of Il27 include potential side effects, preparation purity and other challenges.

Which scientific research fields are deeply studying Il27?

The potential application of Il27 in tumor immunotherapy and autoimmune diseases is being deeply studied in the scientific research field.

Is the production of Il27 related to genetic factors?

The production of Il27 is related to genetic factors, and the expression level and polymorphism of genes may affect its production and function.

Which role of Il27 in inflammatory diseases?

Studies have shown that Il27 may have an anti-inflammatory effect in inflammatory diseases, regulating the degree of inflammatory response.

What the potential applications of recombinant Il27 in cancer therapy?

Current studies have shown that Il27 has potential application value in enhancing anti-tumor immunity and alleviating tumor invasion and metastasis.

What is the effect of Il27 on immune response?

Il27 can enhance the activation and proliferation of specific immune cells and regulate the intensity and direction of immune response.