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Recombinant Mouse Dihydrofolate Reductase, His-tagged

Cat.No. : Dhfr-3422M
Product Overview : Recombinant mouse DHFR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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Cat. No. : Dhfr-3422M
Description : Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.
Source : E.coli.
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
Molecular Weight : 23.8 kDa (207aa) confirmed by MALDI-TOF.
Purity : > 95% by SDS - PAGE.
Concentration : 1 mg/ml (determined by Bradford assay)
Sequences Of Amino Acids : MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EF KYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KE PP RG AHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HL RL FV TRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD.
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways : Metabolic pathways; Folate biosynthesis; One carbon pool by folate
Gene Name : Dhfr dihydrofolate reductase [ Mus musculus ]
Synonyms : dihydrofolate reductase; AA607882; AI662710; AW555094; 8430436I03Rik; Dhfr
Gene ID : 13361
mRNA Refseq : NM_010049
Protein Refseq : NP_034179
UniProt ID : P00375
Chromosome Location : 13 C3; 13 43.0 cM
Function : NADP or NADPH binding; dihydrofolate reductase activity; dihydrofolic acid binding; drug binding; oxidoreductase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can you name a common DHFR inhibitor drug used in cancer treatment? 03/12/2020

Methotrexate is a common DHFR inhibitor drug used in cancer treatment.

Are there ongoing research efforts to develop new DHFR inhibitors for clinical use? 02/21/2018

Yes, scientists continue to research and develop new DHFR inhibitors with improved efficacy and reduced side effects for various clinical applications.

Are there any drug-resistant strains that have developed due to DHFR inhibitors? 12/06/2017

Yes, drug-resistant strains have emerged due to mutations in DHFR genes, reducing the effectiveness of DHFR inhibitor drugs.

Are there any side effects associated with DHFR inhibitor drugs like Methotrexate? 11/28/2017

Yes, common side effects of DHFR inhibitors include nausea, vomiting, decreased blood cell counts, and liver toxicity.

Can you name an antibiotic that inhibits DHFR in bacteria? 11/21/2016

Trimethoprim is an antibiotic that inhibits DHFR in bacteria.

Customer Reviews (3)

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Reviews
04/16/2023

    The DHFR protein offered by the manufacturer is of exceptional quality, making it an ideal choice to fulfill my experimental needs.

    03/20/2017

      Another crucial aspect where the manufacturer excels is their efficient supply chain management.

      08/31/2016

        The technical support provided by the manufacturer is invaluable in overcoming experimental hurdles and optimizing the utilization of DHFR protein.

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