| Species : |
Mouse |
| Source : |
Mammalian Cells |
| Tag : |
His |
| Protein Length : |
826-1816 a.a. |
| Description : |
Laminin subunitalpha-4 is a protein that in humans is encoded by the LAMA4 gene. Laminins, afamily of extracellular matrix glycoproteins, are the major noncollagenousconstituent of basement membranes. They have been implicated in a widevariety of biological processes including cell adhesion, differentiation,migration, signaling, neurite outgrowth and metastasis. Laminins are composedof 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, andB2, respectively) and they form a cruciform structure consisting of 3 shortarms, each formed by a different chain, and a long arm composed of all 3chains. Each laminin chain is a multidomain protein encoded by a distinctgene. Several isoforms of each chain have been described |
| MolecularMass : |
112.0 kDa |
| Purity : |
>90%, by SDS-PAGEunder reducing conditions and visualized by silver stain. |
| Activity : |
Measured by theability of the immobilized protein to support the adhesion of HT1080 humanfibrosarcoma cells.The ED50 for this effect is typically 1-4μg/mL. |
| EndotoxinLevel : |
<1.0 EU per 1 μgof the protein by the LAL method. |
| N-terminalSequenceAnalysis : |
Mouse myeloma cellline, NS0 derived Gln826-Ala1816,with a Cterminal10 His tag |
| Formulation : |
Lyophilized from a0.2 μm filtered solution in Tris and NaCl, pH 6.0. |
| Reconstitution : |
Reconstitute at 200μg/mL in PBS. |
| Storage : |
Use a manual defrostfreezer and avoid repeated freeze thaw Cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution. |
