Active Recombinant Human GRK5, GST-tagged

• Cat.No.: GRK5-1410H
• Product Overview: Recombinant full-length human GRK5 was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Specification

• Description: GRK5 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. It phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. GRK5 plays a role in regulating the motility of polymorphonuclear leukocytes (PMNs). Desensitization of G protein-coupled receptors regulates the number of polymorphonuclear leukocytes (PMNs), as well as their motility and ability to stop upon contact with pathogens or target cells, and this desensitization is mediated by GRK5.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 40 nmol/min/mg
• Molecular Mass: ~95 kDa
• Protein length: Full length
• Purity: >90%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: GRK5 G protein-coupled receptor kinase 5 [ Homo sapiens ]
• Official Symbol: GRK5
• Synonyms: GRK5; G protein-coupled receptor kinase 5; GPRK5; FP2025; g protein-coupled receptor kinase GRK5; FLJ39780
• Gene ID: 2869
• mRNA Refseq: NM_005308
• Protein Refseq: NP_005299
• MIM: 600870
• UniProt ID: P34947
• Chromosome Location: 10q24-qter
• Pathway: Alpha-synuclein signaling, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; G alpha (q) signalling events, organism-specific biosystem
• Function: ATP binding; G-protein coupled receptor kinase activity; nucleotide binding; phospholipid binding; protein binding; protein kinase C binding; protein serine/threonine kinase activity