| Description : |
GDF-15 belongs to the TGF-β cytokine family whose members play an important role during prenatal development and postnatal growth, remodeling and maintenance of a variety of tissues and organs. GDF-15 is expressed predominantly in placenta and to a much lesser extent in various other tissues. The presence of GDF-15 in amniotic fluid and its elevated levels in the sera of pregnant women suggest a role for GDF-15 in gestation and embryonic development. GDF-15 generally exerts tumor suppressive activities and is one of the predominant factors produced and secreted in response to activation of the p53 pathway. Interestingly, the serum level of GDF-15 is positively correlated with neoplastic progression of several tumor types, including certain colorectal, pancreatic, and prostate cancers. Human GDF-15/MIC-1 is a disulfide linked homodimeric protein consisting of two 114 amino-acid polypeptide chains. |
| Source : |
Cell Culture |
| Species : |
Human |
| Form : |
Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM sodium citrate, pH 3.0. |
| Bio-activity : |
Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3/E1 osetoblast cells. The expected ED50 for this effect is 1.0-3.0 μg/ml. |
| AA Sequence : |
ARNGDHCPL GPGRCCRLH TVRASLEDL GWADWVLSP REVQVTMCI GACPSQFRA ANMHAQIKT SLHRLKPDT VPAPCCVPA SYNPMVLIQ KTDTGVSLQ TYDDLLAKD CHCI |
| Endotoxin : |
< 1EU per 1µg (determined by the LAL method) |
| Purity : |
Above 95% as determined by SDS-PAGE Analysis. |
| Storage : |
Store at -20 centigrade |
| Reconstitution : |
Centrifuge the vial prior to opening. Rconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein(example 0.1% BSA) and store in working aliquots at -20 centigrade to -80 centigrade. |
