Active Recombinant Mouse Interleukin 16
Cat.No. : | Il16-486M |
Product Overview : | Mouse IL-16 is comprised of 127 amino acids with a molecular weight of 13.2 kDa |
- Specification
- Gene Information
- Related Products
Cat. No. : | Il16-486M |
Description : | Mouse IL-16 was originally described as Lymphocyte Chemoattractant Factor (LCF), is produced primarily by CD4 (+) and CD8 (+) T cells and acts as a chemoattractant for lymphocytes, monocytes, eosinophils, dendritic cells and Langerhanscells. Additionally, IL-16 has been reported to upregulate IL-2 receptor (CD25), induce progression of cells to the G (1) phase and suppresses HIV & SIV replication. Other cells which have been shown to express IL-16 are; B-cells, eosinophils, macrophages, epithelial cells, fibroblasts, microglial cells and keratinocytes. Human and Mouse IL-16 share a high degree of structural and functional similarity. |
Species : | Mouse |
Source : | E.coli |
Form : | Lyophilized at a concentration of 1 mg/ml in 10mM NaP, PH 7.5 |
Purity : | Purity Greater than 97% as determined by: Reducing and non-reducing SDS-PAGE. |
Bio-activity : | Recombinant mouse IL-16 is fully biologically active compared to standard. The ED50, as determined by dose-dependent chemotactic induction of mouse CD4+ T-cells, is 200ng/ml. |
Physical Appearance : | Sterile Filtered white lyophilized (freeze-dried) powder. |
Solubility : | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 - 0.5 mg"s/ml, which can be further diluted into other aqueous solutions. |
Purity Greater than 97% as determined by : | Reducing and non-reducing SDS-PAGE. |
Protein Content determined by : | UV spectroscopy at 280 nm. Quantitation on SDS-PAGE against a known standard. |
Endotoxin Level : | Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug. |
Amino acid sequence : | MHDLNSSTDS AASASAASDI SVESKEATVC TVTLEKTSAG LGFSLEGGKG SLHGDKPLTI NRIFKGDRTGEMVQPGDEIL QLAGTAVQGL TRFEAWNVIK ALPDGPVTIV IRRTSLQCKQ TTASADS |
Storage : | Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. |
Gene Name : | Il16 interleukin 16 [ Mus musculus ] |
Official Symbol : | Il16 |
Synonyms : | Il16; interleukin 16; KIAA4048; mKIAA4048 |
Gene ID : | 16170 |
mRNA Refseq : | NM_010551 |
Protein Refseq : | NP_034681 |
UniProt ID : | O54824 |
Chromosome Location : | 7 D2-D3; 7 41.2 cM |
Function : | cytokine activity; protein binding |
Products Types
◆ Recombinant Protein | ||
IL16-739H | Active Recombinant Human IL16 protein(Pro2-Ser130), His-tagged | +Inquiry |
IL16-136M | Recombinant Mouse IL16 Protein | +Inquiry |
Il16-51M | Recombinant Mouse Il16 Protein (His499-Ser624), C-His tagged, Animal-free, Carrier-free | +Inquiry |
IL16-2865H | Recombinant Human IL16 protein(1211-1330 aa), C-His-tagged | +Inquiry |
IL16-1007C | Recombinant Chicken IL16 Protein, His-tagged | +Inquiry |
◆ Native Protein | ||
IL16-29736TH | Native Human IL16 | +Inquiry |
◆ Lysates | ||
IL16-5245HCL | Recombinant Human IL16 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (10)
Ask a questionLeukocyte migration assays measure IL16's chemotactic impact on cells.
Flow cytometry and qPCR assess T cell activation markers affected by IL16.
Gene expression arrays reveal IL16's influence on inflammatory gene profiles.
RNA interference used to knock down IL16 expression in cell lines.
Experimental setup for studying IL16's modulation of HIV infection involves viral assays.
Binding affinity of IL16 to CD4 receptors determined through SPR or ITC.
Flow cytometry and confocal microscopy visualize IL16's cellular localization.
Mouse models and clinical samples aid in understanding IL16's role in autoimmunity.
ELISA quantifies secreted IL16 levels from cell culture supernatants.
Strategies like animal models and patient samples explore IL16's role in allergies.
Customer Reviews (3)
Write a reviewSuitable for ELISA.
High specificity in ELISA.
Potent in chemotaxis studies.
Ask a Question for All Il16 Products
Required fields are marked with *
My Review for All Il16 Products
Required fields are marked with *
Inquiry Basket